Qualitative and Quantitative Analyses of Polar Glycerolipids in Chlamydomonas reinhardtii based on LC-MS Techniques
Received date: 2017-09-24
Online published: 2018-12-05
The polar glycerolipidome of the model algal strain Chlamydomonas reinhardtii was analyzed qualitatively and quantitatively by using liquid chromatography coupled with mass spectrometry (LC-MS). Based on UPLC-ESI-Q-Trap/MS and UPLC-ESI-Orbitrap/MS2, MS scanning, including neutral loss or precursor ion scanning, and MS/MS scanning were used to identify 109 molecular species possessing distinct mass-to-charge ratios and acyl chains. Targeted quantitative analyses based on external standards with UPLC-ESI-Q-Trap/MS in multiple reactions monitoring (MRM) mode was used to analyze individual molecular species. The results indicated that the glycerolipids of C. reinhardtii mainly included glycolipids (i.e., MGDG and DGDG) and betaine lipid, DGTS. In addition, analysis of fatty acyl constituents of all polar membrane lipids revealed that DGDG, SQDG, DGTS and PI acted as carriers for desaturation of C18 fatty acids. This study established a platform for characterizing the structure spectra and quantitative analysis with LC-MS techniques for C. reinhardtii, which provides a useful tool for studies of the biological function of polar membrane lipids and lipid metabolism in microalgae.
Yang Miao, Meng Yingying, Chu Yadong, Xue Song . Qualitative and Quantitative Analyses of Polar Glycerolipids in Chlamydomonas reinhardtii based on LC-MS Techniques[J]. Chinese Bulletin of Botany, 2018 , 53(6) : 812 -828 . DOI: 10.11983/CBB17179
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