Bioinformatics Analysis of BnMYB80 Genes in Brassica napus
Received date: 2016-01-11
Accepted date: 2016-04-26
Online published: 2018-08-10
MYB transcription factors play a key role in anther development and pollen formation in higher plants, and MYB80 is an important transcription factor involved in the development of tapetum and initiation of male sterility. In this study, we performed BLASTN and TBLASTX searches of the Arabidopsis thaliana MYB80 (AtMYB80) amino acid sequences against the published database and identified 2, 2 and 6 homologous sequences of MYB80 gene in Brassica rapa, B. oleracea and B. napus, respectively. The composition of nucleic acid sequence and amino acid sequences, subcellular localization, phosphorylation sites, hydrophilicity and hydrophobicity, secondary and tertiary structure of protein, conserved domains of the MYB80 were predicted and analyzed by bioinformatics tools and resources from the Internet. The results showed that the MYB80 transcription factors are located in the nucleus by analyzing subcellular localization, with a number of different phosphorylation sites, and the peptide chains are hydrophilia. The predicted secondary structure and tertiary structure showed that the main structural elements of the MYB80 protein were α helix and random coil. Conserved domain analysis indicated that the MYB80 transcription factor belongs to the MYB-R2R3 type transcription factor, with 2 tandem SANT domains in the N-terminal sequence. The multiple sequence ratio and phylogenetic tree were constructed with MYB80 proteins from different plants; similarity among B. napus and B. rapa, and B. oleracea was more than 92%, and the conserved functional domains had high homology and strong sequence conservation. These results are helpful for resolving the biological functions of MYB80 and the molecular mechanism of sterility regulation and lay a foundation for the heterosis utilization in B. napus.
Key words： bioinformatics; Brassica napus; MYB80; pollen development; tapetum
Jia Ledong, Li Shimeng, Xu Daixiang, Qu Cunmin, Li Jiana, Wang Rui . Bioinformatics Analysis of BnMYB80 Genes in Brassica napus [J]. Chinese Bulletin of Botany, 2016 , 51(5) : 620 -630 . DOI: 10.11983/CBB16006
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