Establishment of Plant Regeneration System from Hairy Roots of Aconitum coreanum
? These authors contributed equally to this paper
Received date: 2014-09-22
Accepted date: 2015-03-30
Online published: 2015-10-09
The hairy roots of Aconitum coreanum were used as explants to induce callus via differentiation in 1/2MS medium with 0.5-5 mg·L-1 6-BA and 0-0.5 mg·L-1 NAA, then regeneration plantlets were induced from the callus. A whole transgenetic plantlet was obtained. The optimal medium for inducing callus and adventitious buds was 1/2MS+2.0 mg·L-1 6-BA+0.2 mg·L-1 NAA+3% sugar. The optimal medium for inducing roots was 1/2MS+0.5 mg·L-1 6-BA+0.1 mg·L-1 NAA+5 mg·L-1 GA+3% sugar.
Lili Liu, Shubo Lu, Jiaping Xu, Qingtian Zhang, Changyu Li . Establishment of Plant Regeneration System from Hairy Roots of Aconitum coreanum[J]. Chinese Bulletin of Botany, 2015 , 50(5) : 623 -6an class="mag_rich_font_size_no_zero">-627 . DOI: 10.11983/CBB14174
1 | 冯欢, 易姝利, 谢佳恒, 雷梦琦, 黄萱 (2014). 微型月季愈伤组织诱导及植株再生. 植物学报 49, 595-602. |
2 | 侯丽丽, 施和平, 余武, 曾宝强, 周卓辉 (2014). 烟草毛状根多倍体诱导及其植株再生. 生物工程学报 30, 581-594. |
3 | 李昌禹, 王英平, 卢淑波, 张庆田, 孔祥义, 徐佳萍, 艾军 (2012). 黄花乌头转基因苗及其获得方法. 中国专利. 2011- 10317204.4. 2012-06-20. |
4 | 李昌禹, 王英平, 杨福合, 卢淑波, 臧埔, 孔长伟, 孔祥义 (2006). 黄花乌头发根及其获得方法. 中国专利. 2006100- 89523.3. 2006-12-27. |
5 | 李昌禹, 王英平, 臧埔, 卢淑波, 宋婷婷 (2005). 黄花乌头叶片诱导植株再生研究. 特产研究 27(2), 34-36. |
6 | 施和平, 朱远锋, 王蓓, 孙蒋兵, 黄胜琴 (2014). 香石竹毛状根诱导、离体培养及其植株再生. 生物工程学报 30, 1742-1750. |
7 | 中国科学院中国植物志编辑委员会 (1979). 中国植物志(第27卷). 北京: 科学出版社. pp. 323. |
8 | Bai YZ, Li CY, Zhao JH, Zheng PH, Li YL, Pan YQ, Wang YP (2013). A high yield method of extracting alkaloid from Aconitum coreanum by pulsed electric field.Chromatographia 76, 635-642. |
9 | ? |
10 | ? … show all 7 hide |
11 | Li CY, Lu SB, Kong XY, Liu LL, Wang YP (2013). Optimization of suspension culture conditions for hairy roots of Aconitum coreanum (Levl.) Raipaics.Agric Biotechnol 2(4), 5-7. |
/
〈 | 〉 |