Chinese Bulletin of Botany >

2015 , Vol. 50 >Issue 5: 623 - 627

DOI: https://doi.org/10.11983/CBB14174

Establishment of Plant Regeneration System from Hairy Roots of Aconitum coreanum

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  • Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China

? These authors contributed equally to this paper

Received date: 2014-09-22

  Accepted date: 2015-03-30

  Online published: 2015-10-09

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Abstract

The hairy roots of Aconitum coreanum were used as explants to induce callus via differentiation in 1/2MS medium with 0.5-5 mg·L-1 6-BA and 0-0.5 mg·L-1 NAA, then regeneration plantlets were induced from the callus. A whole transgenetic plantlet was obtained. The optimal medium for inducing callus and adventitious buds was 1/2MS+2.0 mg·L-1 6-BA+0.2 mg·L-1 NAA+3% sugar. The optimal medium for inducing roots was 1/2MS+0.5 mg·L-1 6-BA+0.1 mg·L-1 NAA+5 mg·L-1 GA+3% sugar.

Cite this article

Lili Liu, Shubo Lu, Jiaping Xu, Qingtian Zhang, Changyu Li . Establishment of Plant Regeneration System from Hairy Roots of Aconitum coreanum[J]. Chinese Bulletin of Botany, 2015 , 50(5) : 623 -6an class="mag_rich_font_size_no_zero">-627 . DOI: 10.11983/CBB14174

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