Research Progress on Plant Secondary Metabolite Biosyn-thetic Gene Clusters

doi:10.11983/CBB22232

Abstract

Abstract: The secondary metabolites produced by plants provide human beings with a wealth of pharmaceutical, perfume and industrial raw materials. With the rapid development of molecular biology and genomics research, the biosynthetic gene clusters (BGCs) of secondary metabolites of various plants have been analyzed, which opens a new path for us to quickly obtain the biosynthetic pathways of target products and discover novel natural products. This paper focuses on the definition and characteristics of plant secondary metabolite biosynthesis gene clusters, and its basic structural models, evolution and regulatory mechanisms, in order to provide theoretical basis and reference for related research.

Key words: secondary metabolites, biosynthetic gene cluster, BGCs regulation, BGCs characteristics

Lu Zhu, Chong Yuan, Yifei Liu. Research Progress on Plant Secondary Metabolite Biosyn-thetic Gene Clusters[J]. Chinese Bulletin of Botany, 2024, 59(1): 134-143.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB22232

https://www.chinbullbotany.com/EN/Y2024/V59/I1/134

Figures/Tables 4

Figure 1 Number of published articles on plant secondary metabolic biosynthetic gene clusters in recent years

Figure 2 Three possible scenarios for the origin of biosynthetic gene clusters in plants (modified from Nützmann et al., 2016)

Figure 3 Four basic structural models of plant secondary metabolite biosynthesis gene clusters (modified from Nützmann et al., 2016) *Signature genes

Figure 4 Genomic clustering of cyanogenic glucoside biosynthetic genes in three typical plants (modified from Takos et al., 2011) Functional genes are presented by arrows indicating their orientation. Confirmed genes in cyanogenic glycoside biosynthesis are labelled above each bar, with CYP79 genes in red, CYP71 genes in orange, CYP736 genes in green, and UGT85 genes in blue. The three CYP736A2-like pseudo-genes are indicated below the Lotus corniculatus bar, as is the additional CYP71 in Sorghum bicolor. The Rho locus is within 2 cM of the CM0241 contig. The remaining genes are numbered and annotated as follows: (1) Acireductone dioxygenase; (2) Nucleic acid binding, OB-fold; (3), (4) Hypothetical proteins; (5) Ribonuclease H; (6) Short-chain dehydrogenase/reductase; (7), (8) Putative transposases; (9) Leucine-rich repeat receptor-like protein kinase; (10), (11) α/β-fold hydrolases; (12) Hypothetical protein; (13)-(15) Putative hydroxynitrile lyases; (16) Hypothetical protein.

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