Chinese Bulletin of Botany ›› 2017, Vol. 52 ›› Issue (6): 783-787.DOI: 10.11983/CBB16246

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Induction of Hairy Roots of Scrophularia buergeriana and Its Plant Regeneration

Ren Ruyi*(), Xue Jukun, Guo Huiyan, Wei Jicheng   

  1. College of Life Sciences and Technology, Mudanjiang Normal University, Mudanjiang 157011, China
  • Received:2016-12-13 Accepted:2017-07-04 Online:2017-11-01 Published:2018-02-22
  • Contact: Ren Ruyi

Abstract: Hairy roots were induced by infecting leaf explants of Scrophularia buergeriana with Agrobacterium rhizo- genes strains A4 and R1601. Hairy roots could grow rapidly and autonomously in liquid or solid plant-growth regulator-free MS medium. The transformation was confirmed by PCR amplification of the rolB gene of the Ri plasmid from S. buergeriana hairy roots. Hairy roots could form green callus in MS medium with 0.5 mg·L-1 6-BA and 0.1 mg·L-1 NAA, then regenerated plantlets were induced from the callus. A whole transgenic plantlet was obtained. Adventitious buds could be directly induced from hairy roots in MS medium with 0.5 mg·L-1 6-BA and 0.02 mg·L-1 NAA after about 15-20 d, with the induction rate of buds up to 85%.

Key words: Scrophularia buergeriana, Agrobacterium rhizogenes, hairy root, plant regeneration