Chinese Bulletin of Botany ›› 2004, Vol. 21 ›› Issue (05): 559-564.

• 技术与方法 • Previous Articles     Next Articles

Regenerated Organs of Cyclamen persicum Mill. and Their Proliferative Stability in Tissue Culture

1QU Fu-Ning② 1YOU Cui-Rong 1GONG Xue-Qin 2WANG Yun-Shan 2KANG Li-Fang   

  1. 1(Yantai University Biochemistry Department, Yantai 264005) 2(Shanxi Province Horticulture, Taiyuan 030031)
  • Received:2003-07-16 Revised:2004-02-25 Online:2004-10-20 Published:2004-10-20
  • Contact: QU Fu-Ning

Abstract: Leaf calli of two cyclamen cultivars, ‘Victoria’ and ‘Dahong’ were inoculated on 12 kinds of media supplemented with of 6-BA and NAA. Five types of regenerated organs appeared, including adventitious bud point, multiple-bud bulb, multiple-leaf new shoot, single-bud bulb and leaf-like bud clump. Tests were made on proliferative stability of different regenerated organs and multiple-bud bulbs and multiple-leaf new shoost of ‘Dahong’ were more stable in continued culture. Compared with multiple-leaf new shoots, multiple-bud bulbs showed high proliferative rate and were ideal for continued culture. Single-bud bulbs and leaf-like bud clumps were abnormal regenerated organs. Multiple-bud bulbs and multiple-leaf new shoots of ‘Victoria’ were instability in continued culture and they were liable to produce abnormal organs.