Abstract: A genetic transfomation regeneration system from aseptic seedling leaves of chrysanthemum (Dendranthema morifolium) was developed. Adventitious shoots were directly induced and regenerated from leaf explants of chrysanthemum, and the medium (MS+6-BA 3 mg.L-1+NAA 1 mg. L-1), which could directly induce shoot regeneration, was used. Shoots were rooted on half-strength MS media with IBA and NAA. The rooted plantlets grew well in greenhouse.