Chinese Bulletin of Botany ›› 2014, Vol. 49 ›› Issue (6): 682-691.DOI: 10.3724/SP.J.1259.2014.00682

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Cloning and Expression of a Proline Dehydrogenase Gene from Celastrus orbiculatus

Lijuan Wei1, Juan Zhang2*, Lei Wang1, Linde Liu1, Tongxin Zhao1, Qingrong Huang1   

  1. 1Life Sciences School of Ludong University, Yantai 264025, China;

    2Agricultural School of Ludong University, Yantai 264025, China
  • Received:2013-09-24 Revised:2014-05-14 Online:2014-11-01 Published:2014-11-21
  • Contact: Juan Zhang

Abstract: We cloned a proline dehydrogenase gene from Celastrus orbiculatus using degenerate primers and RACE and named it NstProDH1. Sequence alignment showed that NstProDH1 has high sequence similarity with corresponding genes from Arabidopsis and tobacco. Enzymatic characteristic analysis identified the activity of proline dehydrogenase. Comparison of gene expression patterns and proline dehydrogenase activity were not associated in different organs, so NstProDH1 was regulated by transcription and translation. The gene expression pattern of NstProDH1 was similar to that of Arabidopsis ProDH1, therefore NstProDH1 may be functionally similar to Arabidopsis ProDH1.

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