Chinese Bulletin of Botany ›› 2022, Vol. 57 ›› Issue (3): 350-357.DOI: 10.11983/CBB21182

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Rapid Propagation Technology and Field Production of Hemerocallis fulva cv. ‘Fenmeiren’

Lü Xiuli1, Yu Zequn2, Chen Xiangbo1, Fu Renjie1, Miao Shanshan3,*(), Du An3,*()   

  1. 1Shanghai Academy of Landscape Architecture Science and Planning, Shanghai 200232, China
    2Shanghai Gardening-Landscaping Construction Co, Ltd, Shanghai 200333, China
    3Shanghai Landscape Architecture Design Research Institute Co, Ltd, Shanghai 200031, China
  • Received:2021-10-22 Accepted:2022-04-20 Online:2022-05-01 Published:2022-05-18
  • Contact: Miao Shanshan,Du An
  • About author:First author contact: These authors contributed equally to this paper.

Abstract: Using the scape as explants, we successfully established the tissue culture and rapid propagation technology for the large-scale production of Hemerocallis fulva cv. ‘Fenmeiren’. Our results showed that the survival rate of explant was 95%, which explants were obtained in June and sterilized in the 15% (v/v) sodium hypochlorite solution for 8 minutes. The best proliferation medium was MS + 1.0 mg·L-1 6-BA + 0.004 mg·L-1 TDZ + 0.1 mg·L-1 NAA. After 30 days of cultivation, the monthly proliferation coefficient reached 2.9. On the medium of MS + 0.1 mg·L-1 6-BA + 0.1 mg·L-1 IBA, the tissue culture seedlings did not differentiate and grew vigorously. The optimal rooting medium was 1/2MS + 0.4 mg·L-1 IBA + 20 g·L-1 sucrose, and the rooting rate was 95%. by use of perlite:peat=1:2 (v/v) as the transplanting matrix, the survival rate of transplanting was 85% and the qualified rate was 75%. At present, the large-scale production of this cultivar has been achieved and 2.0 × 105 tissue culture seedlings were produced, which all performed well in the field.

Key words: Hemerocallis fulva cv. ‘Fenmeiren’, rapid propagation technology, large-scale production, field planting