Construction of a Genetic Map and QTL Mapping of Fruit-related Traits in Eggplant (Solanum melongena)

doi:10.11983/CBB16003

Abstract

Abstract: A linkage map of eggplant (Solanum melongena) was constructed for an intraspecific RIL population derived from a cross between 09-101-M and 10TL-102-F₄-1. The map contains 157 markers, encompasses 16 linkage groups, spanning 991.7 cM and spaced at an average interval of 6.32 cM. Composite interval mapping analysis with genotypic data from 157 molecular markers revealed the positions of 18 quantitative trait loci (QTL) for fruit-related traits. Ten of the QTLs identified were major QTLs, and 8 QTLs in corresponding positions were detected in both experimental locations and years. In all QTLs, the effect value of QTL fw1.1, located on LG01 (E09) E25M34-E33M57b and controlling fruit weight was the largest, reaching 23.8% to 31.6%. Fruit length and fruit diameter were significantly correlated with fruit weight, and the QTLs controlling fruit length, fruit diameter and fruit weight were in similar map positions.

Lifeng Xie, Ye Li, Jingfu Li, Ning Li. Construction of a Genetic Map and QTL Mapping of Fruit-related Traits in Eggplant (Solanum melongena)[J]. Chinese Bulletin of Botany, 2016, 51(5): 601-608.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB16003

https://www.chinbullbotany.com/EN/Y2016/V51/I5/601

Figures/Tables 5

References 24

1	曹必好, 雷建军, 孙秀东, 陈国菊, 孟成民 (2006). 茄子 RAPD分子标记图谱的构建. 园艺学报 33, 1092-1092.
2	黄锐明, 陈国良, 谢晓凯, 卢永奋, 卢海强 (2006a). 茄子果径遗传效应初探. 长江蔬菜 9, 45-46.
3	黄锐明, 谢晓凯, 卢永奋, 卢海强, 柳江海 (2006b). 茄子果长遗传效应的初步研究. 广东农业科学 7, 25-26.
4	黄晓梅, 王傲雪, 许向阳, 李景富, 李宁 (2010). 番茄遗传图谱构建和抗晚疫病基因Ph-2的QTL分析. 园艺学报 37, 1085-1092.
5	李怀志 (2011). 茄子遗传连锁图谱构建及果实相关性状QTL 定位. 博士论文. 上海: 上海交通大学. pp. 65-66.
6	李宁, 李烨, 姚明华, 谢立峰, 张环宇 (2015). 茄子遗传图谱构建及重要性状分子定位的研究进展. 分子植物育种 9, 2127-2134.
7	李锡香, 朱德蔚 (2006). 茄子种质资源描述规范和数据标准. 北京: 中国农业出版社. pp. 11-21.
8	刘进生, Phatak SC (1992). 茄子功能性雄性不育的遗传及其与果紫色基因连锁关系的研究. 遗传学报 19, 349-354.
9	乔军, 陈钰辉, 王利英, 刘富中, 张映, 连勇 (2012). 茄子果形的QTL定位. 园艺学报 39, 1115-1122.
10	乔军, 刘富中, 陈钰辉, 连勇 (2011a). 茄子果萼色遗传研究. 植物遗传资源学报 12, 806-810.
11	乔军, 刘富中, 陈钰辉, 连勇 (2011b). 茄子果形遗传研究. 园艺学报 38, 2121-2130.
12	许向阳, 孟凡娟, 李景富 (2007). 番茄资源对叶霉病的抗性鉴定和SSR标记遗传变异分析. 植物病理学报 37, 520-527.
13	Food and Agriculture Organization of the United Nations (FAO) (2013). FAO STAT. .
14	Foolad MR (1999). Comparison of salt tolerance during seed germination and vegetative growth in tomato by QTL mapping.Genome 42, 727-734.
15	Frary A, Doganlar S, Daunay MC, Tanksley SD (2003). QTL analysis of morphological traits in eggplant and implications for conservation of gene function during evolution of solanaceous species.Theor Appl Genet 107, 359-370.
16	Frary A, Frary A, Daunay MC, Huvenaars K, Mank R, Doğanlar S (2014). QTL hotspots in eggplant (Solanum melongena) detected with a high resolution map and CIM analysis.Euphytica 197, 211-228.
17	Fukuoka H, Miyatake K, Nunome T, Negoro S, Shirasawa K, Isobe S, Asamizu E, Yamaguchi H, Ohyama A (2012). Development of gene-based markers and construction of an integrated linkage map in eggplant by using Solanum orthologous (SOL) gene sets.Theor Appl Genet 125, 47-56.
18	Hirakawa H, Shirasawa K, Miyatake K, Nunome T, Negoro S, Ohyama A, Yamaguchi H, Sato S, Isobe S, Tabata S, Fukuoka H (2014). Draft genome sequence of eggplant (Solanum melongena L.): the representative solanum species indigenous to the old world.DNA Res 21, 649-660.
19	Janick J, Topoleski LD (1963). Inheritance of fruit color in eggplant (Solanum melongena).Proc Am Soc Hort Sci 83, 547-558.
20	Kosambi DD (1943). The estimation of map distances from recombination values. Annu Eugenics 12, 172-175.
21	Nunome T, Ishiguro K, Yoshida T, Hirai M (2001). Mapping of fruit shape and color development traits in eggplant (Solanum melongena L.) based on RAPD and AFLP markers.Breeding Sci 51, 19-26.
22	Nunome TNS, Izumi K, Kanamori H, Koji M, Hirotaka Y, Akio O, Hiroyuki F (2009). Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.).Theor Appl Genet 119, 1143-1153.
23	Van Ooijen JW (2006). JoinMap® 4.0: Software for the Calculation of Genetic Linkage Maps in Experimental Population. Wageningen, Kyazma B. V.
24	Voorrips RE (2002). MapChart: software for the graphical presentation of linkage maps and QTLs.J Hered 93, 77-78.

Traits	Investigation standard
Fruit length (fl)	Length of 10 fruits per genotype were measured (in cm), and the mean fl for each genotype was used for analysis
Fruit diameter (fd)	Width of 10 fruits per genotype were measured (in cm), and the mean fd for each genotype was used for analysis
Fruit shape index (fsi)	Fruit shape index was calculated as the ratio between length and diameter (fl/fd)
Fruit weight (fw)	Weight of 10 fruits per genotype were measured (in g), and the mean fw for each genotype was used for analysis
Fruit anthocyanin intensity (fai)	Fruit anthocyanin intensity was scored the degree of pigmentation in the purple fruits only on a scale of 1 to 7 (1=green-white; 3=light purple; 5=purple; 7=dark purple)
Fruit calyx anthocyanin intensity (fcai)	Fruit calyx anthocyanin intensity was evaluated on a 1 to 7 scale like fai

Traits	Investigation standard
Fruit length (fl)	Length of 10 fruits per genotype were measured (in cm), and the mean fl for each genotype was used for analysis
Fruit diameter (fd)	Width of 10 fruits per genotype were measured (in cm), and the mean fd for each genotype was used for analysis
Fruit shape index (fsi)	Fruit shape index was calculated as the ratio between length and diameter (fl/fd)
Fruit weight (fw)	Weight of 10 fruits per genotype were measured (in g), and the mean fw for each genotype was used for analysis
Fruit anthocyanin intensity (fai)	Fruit anthocyanin intensity was scored the degree of pigmentation in the purple fruits only on a scale of 1 to 7 (1=green-white; 3=light purple; 5=purple; 7=dark purple)
Fruit calyx anthocyanin intensity (fcai)	Fruit calyx anthocyanin intensity was evaluated on a 1 to 7 scale like fai

Traits	09-101	10TL-102	F₁
fl	39.2±0.92 a	6.4±0.74 b	22.1±4.12 a
fd	3.0±0.08 a	5.2±0.11 b	4.2±0.42 a
fsi	13.5±0.87 a	1.18±0.78 b	6.6±0.78 a
fw	140±2.12 a	45±0.98 b	96.1±14.1 b
fai	7±0.01 a	1±0.01 b	4.1±1.02 a
fcai	7±0.01 a	1±0.01 b	3.82±1.13 a

Traits	09-101	10TL-102	F₁
fl	39.2±0.92 a	6.4±0.74 b	22.1±4.12 a
fd	3.0±0.08 a	5.2±0.11 b	4.2±0.42 a
fsi	13.5±0.87 a	1.18±0.78 b	6.6±0.78 a
fw	140±2.12 a	45±0.98 b	96.1±14.1 b
fai	7±0.01 a	1±0.01 b	4.1±1.02 a
fcai	7±0.01 a	1±0.01 b	3.82±1.13 a

Traits	fl	fd	fsi	fw	fai
fd	0.498**
fsi	0.803**	0.632**
fw	0.892**	0.843**	0.376*
fai	0.010	-0.128	-0.014	0.011
fcai	0.007	0.009	0.035	0.102	0.113