Chinese Bulletin of Botany ›› 2008, Vol. 25 ›› Issue (05): 579-584.
• 实验简报 • Previous Articles Next Articles
Xiuxia He, Yuanhua Yu*, Yong Zhang
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Abstract: A plant expression vector consisting of PSY gene was constructed and transformed into ginseng callus via Agrobacterium tumefaciens-mediated transformation. PCR and Southern blotting revealed the successful transformation and expression of PSY in the ginseng callus cells. Analysis with thin-layer chromatography (TLC), spectral characterization, as well as high-performance liquid chromatography (HPLC) showed that the amount of b-carotene production was 143 ug.g-1 (dried ginseng callus cells). This provides a good model for improving nutrition in plants by genetic engineering.
Xiuxia He;Yuanhua Yu*;Yong Zhang. Genetic Transformation of PSY Gene in Ginseng[J]. Chinese Bulletin of Botany, 2008, 25(05): 579-584.
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