Abstract: The plant GH3 gene family is a typical auxin-responsive gene family, which is involved in plant growth and development.GH3 genes are involved in the auxin signaling pathway, light signaling and plant defense responses. GH3 proteins conjugate auxin with amino acids, which is important to maintain auxin homeostasis. In this review, we will summarize the recent progress in the structural and functional analysis of the GH3 gene family in plants, especially in Arabidopsis.