Chinese Bulletin of Botany ›› 2008, Vol. 25 ›› Issue (03): 332-336.

• 技术与方法 • Previous Articles     Next Articles

Agarose Blotting: a Simple Way to Observe Plant Epidermal Cells

Jie Wu, Minting Liang, Xiaojing Wang, Jianzong Peng*   

  1. College of Life Sciences, South China Normal University, Guangzhou 510631, China
  • Received:2007-11-26 Revised:2008-01-27 Online:2008-05-01 Published:2008-05-01
  • Contact: Peng Jianzong

Abstract: We describe a new method, agarose blotting, used to detect epidermal cell size and number of plant organs. With this method, clear images of epidermal cell shape can be observed through several steps of fixation, agarose embedding, and agarose cutting by using the plasticity of agarose. This method is easy to use, saves time, is widely applicable and produces accurate results with a clear picture, in compiling statistics of cell size and number during the process of plant development.