Chinese Bulletin of Botany ›› 2007, Vol. 24 ›› Issue (03): 425-432.

Special Issue: 生殖生物学专辑 (2007年24卷3期)

• 研究简报 • Previous Articles     Next Articles

Megasporogenesis, Microsporogenesis and Development of Female and Male Gametophyte of Bupleurum chinense

Yanjie Wang, Jiaheng Shen   

  1. Department of Biology, Institute of Life and Environment Science, Harbin Normal University, Harbin 150080, China
  • Received:2006-07-18 Revised:2006-11-15 Online:2007-05-01 Published:2007-05-01
  • Contact: Jiaheng Shen

Abstract: Paraffin wax specimens of Bupleurum chinense DC. were used to observe the whole process of embryo development, including megasporgenesis, microsporgenesis, and the development of female and male gametophytes. The anther is tetrasporangiate and its wall conforms to the Dicotyledonous type developmentally. The anther wall includes 4 layers: epiderm, endothecium, middle layer and tapetum. Simultaneous cytokinesis in the microspore mother cells follows meiosis, and the microspore tetrads are tetrahedral. Pollen grains are 3 celled when shed. The ovule is anatropous, unitegmic and tenuinucellar. Usually one archesporium functions directly as a megaspore mother cell. Cytokinesis in the megaspore mother cell usually accompanies meiosis, and the chalazal megaspore of a linear or T-shape tetrad develops into a Polygonum-type embryo sac. The innermost layer of the integument differentiates as an endothelium tapetum. In the same bud, the stamen develops earlier than the pistil. We compared the bud size and development stage of pistils and stamens.