Chinese Bulletin of Botany ›› 2006, Vol. 23 ›› Issue (6): 703-707.
• 技术与方法 • Previous Articles Next Articles
Jinya Guo, Yuliang Dong, Jing Yang, Jie Xu, Hanping Yin, Qiulian Zeng
Received:
Revised:
Online:
Published:
Contact:
Abstract: By reducing the length of diethylaminoethanol-cellulose and increasing the pH in elution solution,we developed an improved method for purifying glycolate oxidase with high isoelectric value (pI > 10.0) from green leaves of Spinacia oleracea, Brassica parachinensis, and Phaseolus vulgaris. Purified glycolate oxidase showed high specific activity (54.6-197.0 U.mg-1) and high pI (>10.0). We achieved 21.6-122.68-fold purification and 4.1%-71.5% yield from crude solution during this purification procedure. SDS-PAGE of the glycolate oxidases revealed a 40-kD band, which indicates that the subunit molecular weight of glycolate oxidases in the 3 different plants was identical.
Jinya Guo;Yuliang Dong;Jing Yang;Jie Xu;Hanping Yin;Qiulian Zeng. Improvement of the Purification Method of Plant Glycolate Oxidase[J]. Chinese Bulletin of Botany, 2006, 23(6): 703-707.
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.chinbullbotany.com/EN/
https://www.chinbullbotany.com/EN/Y2006/V23/I6/703