Chinese Bulletin of Botany ›› 2006, Vol. 23 ›› Issue (6): 665-669.

• 研究论文 • Previous Articles     Next Articles

The Transformation and Expression of HBsAg Gene in Arachis hypogaea

Jianguang Zhu, Yahui Wei, Zhiguang Guo, Yizhou Wu, Haoyong Hao, Jie Sun   

  1. Northwest University, Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Xi’an 710069
  • Online:2006-11-20 Published:2006-11-20

Abstract: A plant expression vector carrying hepatitis B surface antigen (HBsAg) gene was constructed and used to transform Arachis hypogaea mediated by Agrobacterium tumefacient. Transgenic Arachis hypogaea plants were identified by PCR and southern-blot. ELISA assay showed that the HBsAg gene was expressed in transgenic Arachis hypogaea and had a expressing level of 2.41μg.g-1 fresh leaves.