Chinese Bulletin of Botany ›› 2005, Vol. 22 ›› Issue (01): 50-53.

• 组织培养简讯 • Previous Articles     Next Articles

Tissue Culture and Rapid Propagation of Nicotiana tabacum ‘K326’

XIE Qing-Hua WU Yi-Xin SANG Lin XIE Shi-Qing   

  1. 1(Technological Research Institute of Biological Resource,Yunnan Normal University, Kunming 650031) 2(Institute of Biotechnology,Yunnan Academy of Agricultural Science, Kunming 650223)
  • Received:2003-09-01 Revised:2003-12-12 Online:2005-02-25 Published:2005-02-25
  • Contact: WU Yi-Xin

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Abstract: Using stems with buds as explants, we studied the optimal tissue culture conditions for the rapid propagation of Nicotiana tobacco ‘K326’. MS medium with 2 mg.L-1 6-BA, 0.2 mg.L-1 NAA, 30 g.L-1 sugar and 6 g.L-1 agar was suitable for callus induction and proliferation of bud clusters. The rate of rooting reached 100% on MS medium with sugar and agar, and the survival rate of the plants reached 100% after transplanting to soil.

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