Abstract: In this work, the effects of culture factors ( MET, IBA, culture medium and culturing in dark) on root formation were studied in tissue culture of Celastrus angulatus. The results showed that the root induction was significantly affected by duration of culture in dark, while other factors were least effective. The order of their efficiencies was: duration of culture in dark＞culture medium ＞MET×IBA＞MET＞IBA.The best culture medium for root induction of Celastrus angulatus was that containing: 1/2 MS+MET3.0 mg.L-1+IBA 0.8 mg.L-1 or 1/2 MS+ MET3.0 mg.L-1 + IBA 0.5mg.L-1 for 12 days, in dark.