Chinese Bulletin of Botany ›› 2004, Vol. 21 ›› Issue (01): 52-60.
• 研究论文 • Previous Articles Next Articles
WANG Ya-Qin, DUAN Zhong-Gang, HUANG Jiang-Kang, LIANG Cheng-Ye
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Abstract: Factors affecting the regeneration of rice plants from young panicles , such as developmental stage of young panicles in different genotypes, sterilizing methods, duration of 4℃ treatment, induction, differentiation and rooting conditions, etc., were investigated. The results showed that the optimal conditions for different genotypic rice were as following: after the best developmental stage ( Ⅲ~Ⅳ of indica,Ⅰ~Ⅱ of japonica,Ⅴ of wide compatible variety, WCV) of young panicles was treated at 4℃ within 5 days, the materials were sterilized with 0.5% potassium hypochlorite for 20 minutes, cultivated in YS-Y medium about 20 days to induce the calli , then transferred to YS-YF、YS-F medium about 15 days to regenerate shoots and YS-SG medium for 1~2 weeks to induce the roots. Under the optimal conditions, the frequency of green shoot differentiation was a little more than 85% (indica), 90% (japonica), 85% (WCV), the frequency of root regenerated was about 115% (indica), 130% (japonica), 115% (WCV), respectively. Therefore a high efficient regeneration system from in vitro culture of young panicles of rice was established for genetic transformation of rice.
WANG Ya-Qin;DUAN Zhong-Gang;HUANG Jiang-Kang;LIANG Cheng-Ye. Efficient Regeneration from In Vitro Culture of Young Panicles of Rice (Oryza sativa L.)[J]. Chinese Bulletin of Botany, 2004, 21(01): 52-60.
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