Chinese Bulletin of Botany ›› 2002, Vol. 19 ›› Issue (06): 746-748.

• 技术与方法 • Previous Articles     Next Articles

Improvement on NaOCl Method Used for the Liable_to_roll_up Leaf Epidermis

HONG Ya-PingCHEN Zhi-Duan   

  1. (Laboratory of Systematic & Evolutionary Botany, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093)
  • Received:2001-12-03 Revised:2002-01-18 Online:2002-11-20 Published:2002-11-20
  • Contact: CHEN Zhi-Duan

Abstract: As we prepare the leaf epidermis for examination under light microscopy using the traditional NaOCl method, sometimes difficulties occur in some materials from different families or genera in which the epidermis rolls up easily after dehydration with 100% Ethanol. This paper introduced a method by using a small piece of cover_glass to cover the epidermis before washing it with Xylene, this way the epidermis could maintain open and flat. The improvement made the technique of epidermal preparation based on NaOCl method more perfect.