Abstract: Application and source of plant haploids were concisely introduced. Hi story of acquiring gynogenetic haploids via in vitro culture was reviewed and advantages of gynogenetic haploids were presented. In vitro inductive effect of gynogenetic haploid depends on genotype and physiological state of donor plants, development stage of embryo sac, pretreatment of in vitro inoculants, flower appendages, medium composition, mode of inoculation and cultivation conditions, etc. Studies on these factors were systematically summarized. For ontogeny of gynogenetic haploids, some sample studies were presented.