Chinese Bulletin of Botany ›› 2002, Vol. 19 ›› Issue (04): 457-461.
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ZHAO Hong LI Ming-Yang PEI Yan DONG Yu-Mei
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Abstract: A herbicide resistance gene bar was transferred into immature embryos of three wheat cultivars by biolistics. As a result,7 transformed plants were obtained from a total 902 bombardment embryos with the transformation frequency of 0.45~1.2% and transformation time was reduced to 3 months or so. PCR and PCR_Southern blot analysis suggested that the foreign genebar had been transferred into wheat plants. The herbicide_resistance test indicated that four plants were totally resistant to PPT and the other three were partially resistant. It suggested that bar gene was expressed in wheat plants.
ZHAO Hong LI Ming-Yang PEI Yan DONG Yu-Mei. Studies on the Transfer of Herbicide-resistance Gene into Cultivated Wheat by Biolistics[J]. Chinese Bulletin of Botany, 2002, 19(04): 457-461.
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