Chinese Bulletin of Botany ›› 2001, Vol. 18 ›› Issue (01): 118-120.
• 技术与方法 • Previous Articles Next Articles
LIN Yue-Hui LI Han-Bing HE Xin-Qiang
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Abstract: It is normally difficult to cut nice sections and prepare good micrographes from the highly_lignified tissues by means of conventional sectioning methods. In this paper, the cryo-sectioning was introduced to overcome the difficulty and it was further proved to be a fast, simple and effective way of section preparation without passing a long process of softening, dehydration and embedding. The crucial steps are to select a proper concentration of glycerin, a suitable temperature of freezing and an appropriate way of section_flatting.
LIN Yue-Hui LI Han-Bing HE Xin-Qiang. Application of Cryo_sectioning Technique in Highly Lignified Tissues[J]. Chinese Bulletin of Botany, 2001, 18(01): 118-120.
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