Abstract: For the stomatal movement, the regulation of carbon metabolism in guard cells is specific. In guard cells (GC), the activity levels of enzymes necessary for carboxylation and regeneration of RUBP were less than those in mesophyll cells (MC). However, guard_cell PEPC, which catalyses the regulated step in malate accumulation, is crucial for stomata. The enzymes required for conversion of PGA to GAP/DHAP we re quite active in GC. Moreover, there is high enzymes activity, predominance of cytochrome pathway and incomplete engagement of alternative in mitochondria. Respiration is the major source of energy for stomatal movement. A review of the regulation and the characteristic of key enzymes in carbon metabolisms and the role of carbon metabolism in guard cells is given here. In addition, the relationship between carbon metabolism in stomatal guard cells and energy metabolism is discussed. There are mighty advances in the study of the relationship between stomatal movement and carbon metabolisms in guard cells.