Chinese Bulletin of Botany ›› 1999, Vol. 16 ›› Issue (06): 701-704.
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QIAO Ai-Min and FU Jia-Rui
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Abstract: Flowering Chinese cabbage seeds of different vigor were obtained by treatment at 40℃ and 100% RH for different days. Genomic DNA was extracted with balanced phenol and chloroform directly from the dry aged seed. RAPD amplification was carded out on the extracted genomic DNA. The extracted genomic DNA samples were large in quantity and identical. DNA bands amplified by primer S208 were clear and bright. It could be concluded that genomic DNA extracted directly from the dry artificial aged seeds of flowering Chinese cabbage could be used for RAPD analysis.
QIAO Ai-Min and FU Jia-Rui. Direct Extraction of Genomie DNA from Dry Artificially-aged Seeds of Flowering Chinese Cabbage and its Application in RAPD Analysis[J]. Chinese Bulletin of Botany, 1999, 16(06): 701-704.
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