Chinese Bulletin of Botany ›› 1995, Vol. 12 ›› Issue (02): 57-62.

• 研究报告 • Previous Articles     Next Articles

Compound starch Gel Electrophoresis for Isozyme Analysis

Wang Zhong-ren Zhang Fang Kan Zhi-feng   

  1. 1(Institute of Botany, the Chinese Academy of Sciences, Beijing 100093) 2(Shihezi Agriculturae College of Xinjiang, Shihezi, Xinjiang 832003)
  • Online:1995-03-15 Published:1995-03-15

Abstract: For overcoming the difficulty of getting hydrolyzed potato starch and making the isozyme analysis with starch gel electropboresis to be easily undertaken, a chemical potato starch (or food potato starch )mixed with soluble starch and some additives was used instead of hydrolyzed potato starch to make gels. The result shows that cooked with 8-- 10/100 of the above mixed starch (in proportion as 5∶3), adding 1/100 agar powder and 2-4/100 sucrose, the "compound starch gel" can be perfectly sliced and stained for many enzymes, such as PGM ,PGI,MDH, AAT, SKDH, 6PGD,IDH,ME etc. for varied kinds of plant materials.