Abstract: The application of microspore culture still produces low frequency of plant regeneration from microspore-derived embryos in many cabbage and broccoli genotypes. To improve the frequency of plant regeneration, we studied the effect of medium, agar concentration, embryoid type and days of culture of microspores on plant regeneration in hybrid F₁ generations of a cabbage, ‘Zhonggan 11’, and a broccoli, ‘TI-111’. B5 medium with 1%–1.25% agar was optimal for germination and regeneration of embryos, and the frequency of plant regeneration from cotyledonary embryos was significantly higher than that from other embryos, especially cotyledonary embryos from the 25-day-cultured microspores. The frequency of plant regeneration for cotyledonary embryos from 25-day-cultured microspores in 8 cultivars of cabbage and broccoli transferred to B5 medium with 1% agar ranged from 77.8% to 97.2%.