Chinese Bulletin of Botany ›› 2009, Vol. 44 ›› Issue (06): 725-727.DOI: 10.3969/j.issn.1674-3466.2009.06.010

• 技术方法 • Previous Articles     Next Articles

Callus Induction and Rapid Propagation of Chaenomeles speciosa

Xiaofeng Fan, Xiaoqiang Guo, Zhaoxia Xiao, Lingxia Liu


  1. Department of Life Sciences, Longdong University, Qingyang 745000, China
  • Received:2008-12-24 Revised:2009-06-22 Online:2009-11-01 Published:2009-11-01
  • Contact: Xiaofeng Fan

Abstract: This experiment used the current stem-segment with shoot apexes and segments as explants in callus induction and rapid propagation of Chaenomeles speciosa. The optimal medium for bud and callus induction was MS+2.0 mg.L-1 6-BA+1.0mg. L-1 NAA, and the optimal propagation medium was MS+1.0 mg.L-1 6-BA+0.05-0.1 mg.L-1 NAA. The proliferation coefficient was 6.59. For callus differentiation, the optimal medium was MS+ 1.0 mg.L-1 6-BA+ 0.05 mg.L-1 NAA, and for rooting, 1/2 MS+1.0 mg. L-1 IAA. The ratio of rooting was 86.7%, and that of survival was more than 90% when small intact plants were transplanted on perlite seedbeds.