Genetic Mapping and Analysis of a Chloroplast Division Mutant cpd111 in Arabidopsis thaliana

doi:10.3724/SP.J.1259.2012.00226

Abstract

Abstract: Ethyl-methane sulfonate mutagenesis revealed a chloroplast division mutant, chloroplast division111 (cpd111), in Arabidopsis thaliana. Genetic analysis indicated that the mutant was controlled by a single recessive gene. Chloroplasts in the mutant were fewer in number and more heterogeneous in size than those in the wild type. The correlation between chloroplast number and cell size was not obvious in the mutant. Map-based cloning revealed a mutation in FtsZ1 responsible for the mutant phenotype. The mutation affected the splicing and stability of FtsZ1 mRNA, caused early translational termination of the protein and resulted in a chloroplast division defect in cpd111. We provide new materials and information for a better understanding of the function of FtsZ1 in chloroplast division.

Xia Liu, Yuefang Gao, Yuhong Shi, Xinzhao Shen, Zhonghua Liu, Hongbo Gao. Genetic Mapping and Analysis of a Chloroplast Division Mutant cpd111 in Arabidopsis thaliana[J]. Chinese Bulletin of Botany, DOI: 10.3724/SP.J.1259.2012.00226.

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References

El-Kafafi el S, Karamoko M, Pignot-Paintrand I, Grunwald D, Mandaron P, Lerbs-Mache S, Falconet D (2008). Developmentally regulated association of plastid division protein FtsZ1 with thylakoid membranes in Arabidopsis thaliana. The Biochemical journal 409, 87-94.
El-Kafafi el S, Mukherjee S, El-Shami M, Putaux JL, Block MA, Pignot-Paintrand I, Lerbs-Mache S, Falconet D (2005). The plastid division proteins, FtsZ1 and FtsZ2, differ in their biochemical properties and sub-plastidial localization. The Biochemical journal 387, 669-676.
Gao H, Kadirjan-Kalbach D, Froehlich JE, Osteryoung KW (2003). ARC5, a cytosolic dynamin-like protein from plants, is part of the chloroplast division machinery. Proceedings of the National Academy of Sciences of the United States of America 100, 4328-4333.
Glynn JM, Froehlich JE, Osteryoung KW (2008). Arabidopsis ARC6 coordinates the division machineries of the inner and outer chloroplast membranes through interaction with PDV2 in the intermembrane space. The Plant cell 20, 2460-2470.
Glynn JM, Yang Y, Vitha S, Schmitz AJ, Hemmes M, Miyagishima SY, Osteryoung KW (2009). PARC6, a novel chloroplast division factor, influences FtsZ assembly and is required for recruitment of PDV1 during chloroplast division in Arabidopsis. The Plant journal 59, 700-711.
Kuroiwa T, Kuroiwa H, Sakai A, Takahashi H, Toda K, Itoh R (1998). The division apparatus of plastids and mitochondria. International review of cytology 181, 1-41.
Lukowitz W, Gillmor CS, Scheible WR (2000). Positional cloning in Arabidopsis. Why it feels good to have a genome initiative working for you. Plant physiology 123, 795-805.
Maple J, Aldridge C, Moller SG (2005). Plastid division is mediated by combinatorial assembly of plastid division proteins. The Plant journal 43, 811-823.
Maple J, Moller SG (2007). Plastid division: evolution, mechanism and complexity. Annals of botany 99, 565-579.
Maple J, Vojta L, Soll J, Moller SG (2007). ARC3 is a stromal Z-ring accessory protein essential for plastid division. EMBO reports 8, 293-299.
McAndrew RS, Froehlich JE, Vitha S, Stokes KD, Osteryoung KW (2001). Colocalization of plastid division proteins in the chloroplast stromal compartment establishes a new functional relationship between FtsZ1 and FtsZ2 in higher plants. Plant physiology 127, 1656-1666.
McAndrew RS, Olson BJ, Kadirjan-Kalbach DK, Chi-Ham CL, Vitha S, Froehlich JE, Osteryoung KW (2008). In vivo quantitative relationship between plastid division proteins FtsZ1 and FtsZ2 and identification of ARC6 and ARC3 in a native FtsZ complex. The Biochemical journal 412, 367-378.
Miyagishima SY, Froehlich JE, Osteryoung KW (2006). PDV1 and PDV2 mediate recruitment of the dynamin-related protein ARC5 to the plastid division site. The Plant cell 18, 2517-2530.
Nakanishi H, Suzuki K, Kabeya Y, Miyagishima SY (2009). Plant-specific protein MCD1 determines the site of chloroplast division in concert with bacteria-derived MinD. Current biology 19, 151-156.
Nakanishi H, Suzuki K, Kabeya Y, Okazaki K, Miyagishima SY (2009). Conservation and differences of the Min system in the chloroplast and bacterial division site placement. Communicative & integrative biology 2, 400-402.
Okazaki K, Kabeya Y, Miyagishima SY (2010). The evolution of the regulatory mechanism of chloroplast division. Plant signaling & behavior 5, 164-167.
Okazaki K, Kabeya Y, Suzuki K, Mori T, Ichikawa T, Matsui M, Nakanishi H, Miyagishima SY (2009). The PLASTID DIVISION1 and 2 components of the chloroplast division machinery determine the rate of chloroplast division in land plant cell differentiation. The Plant cell 21, 1769-1780.
Osteryoung KW, Stokes KD, Rutherford SM, Percival AL, Lee WY (1998). Chloroplast division in higher plants requires members of two functionally divergent gene families with homology to bacterial ftsZ. The Plant cell 10, 1991-2004.
Raven JA, Allen JF (2003). Genomics and chloroplast evolution: what did cyanobacteria do for plants? Genome biology 4, 209.
Ross J (1995). mRNA stability in mammalian cells. Microbiological reviews 59, 423-450.
Shimada H, Koizumi M, Kuroki K, Mochizuki M, Fujimoto H, Ohta H, Masuda T, Takamiya K (2004). ARC3, a chloroplast division factor, is a chimera of prokaryotic FtsZ and part of eukaryotic phosphatidylinositol-4-phosphate 5-kinase. Plant & cell physiology 45, 960-967.
Stokes KD, McAndrew RS, Figueroa R, Vitha S, Osteryoung KW (2000). Chloroplast division and morphology are differentially affected by overexpression of FtsZ1 and FtsZ2 genes in Arabidopsis. Plant physiology 124, 1668-1677.
Vitha S, Froehlich JE, Koksharova O, Pyke KA, van Erp H, Osteryoung KW (2003). ARC6 is a J-domain plastid division protein and an evolutionary descendant of the cyanobacterial cell division protein Ftn2. The Plant cell 15, 1918-1933.
Yang Y, Glynn JM, Olson BJ, Schmitz AJ, Osteryoung KW (2008). Plastid division: across time and space. Current opinion in plant biology 11, 577-584.
Yoshida Y, Kuroiwa H, Misumi O, Nishida K, Yagisawa F, Fujiwara T, Nanamiya H, Kawamura F, Kuroiwa T (2006). Isolated chloroplast division machinery can actively constrict after stretching. Science 313, 1435-1438.