GATL12 is Essential for Chloroplast Biogenesis in Arabidopsis

doi:10.3724/SP.J.1259.2011.00379

Abstract

Abstract: Glutamine amidotransferase (GATase) catalyses the removal of the ammonia group from glutamine and then transfers this group to a substrate to form a new carbon-nitrogen group. Two classes of GATase domains have been identified: Class I (also known as trpG-type or triad) and Class II (also known as purF-type or Ntn). Arabidopsis genome contains 13 genes encoding Class-I-like proteins (GATLs) and their biological functions have not been reported. We isolated two T-DNA insertion mutants of GATL12 (gatl12-1 and gatl12-2) in which most of the ovules in the heterozygous mutants turned green on day 8 due to the chloroplast accumulation, but about 25% of the ovules were white. About 25% of heterozygous plant seeds cultured in 1/2MS medium developed into albino seedlings, which were homozygous mutants demonstrated by PCR. The transcripts of GATL12 could not be detected in the albino seedlings. Electron microscopy analysis showed that the chloroplasts could not develop in the mutants. Our results suggest that GATL12 plays an important role in chloroplast development in Arabidopsis.

Yanping Su, Xiaoduo Lu, Songdong Shen, Chunyi Zhang. GATL12 is Essential for Chloroplast Biogenesis in Arabidopsis[J]. Chinese Bulletin of Botany, 2011, 46(4): 379-385.

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