Research Advances of the Plant ESCRT Machinery

doi:10.11983/CBB22038

Abstract

Abstract: In eucaryotic cells, the endosomal sorting complex required for transport (ESCRT) machinery, consisting of the conserved multiple sub-complexes, plays important roles in both endocytic and secretory pathways. In plant cells, the ESCRT machinery consists of four ESCRT complexes (ESCRT-I, -II, -III, and the VPS4/SKD1 complex) and several ESCRT accessory proteins, but lacks of the ESCRT-0. Every ESCRT complex contains several subunit proteins. Recent researches have demonstrated that the ESCRT machinery plays an essential role in the vacuolar sorting of ubiquitinated membrane proteins via regulation the biogenesis of multivesicular body (MVB)/PVC (prevacuolar compartment) and autophagic pathway. Recent researches have demonstrated that the ESCRT machinery plays an essential role in the vacuolar sorting of ubiquitinated membrane proteins via regulation the biogenesis of multivesicular body (MVB)/PVC (prevacuolar compartment) and autophagosome as well as response to environmental stresses. In this review, we firstly introduce the subunits and biological functions of the ESCRT machinery in plants, and then summarize the latest research advances on the plant-specific ESCRT complex components. Finally, we discuss future study on the unsolved scientific problems of the ESCRT complex in plants.

Key words: ESCRT complex, MVB/PVC, endocytosis

Wu Fan, Shen Jinbo, Hu Shuai. Research Advances of the Plant ESCRT Machinery[J]. Chinese Bulletin of Botany, 2022, 57(5): 697-712.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB22038

https://www.chinbullbotany.com/EN/Y2022/V57/I5/697

Figures/Tables 5

References 64

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复合体	基因	功能	参考文献
ESCRT-0	N.I.
	VPS23A (AT3G12400)	膜蛋白内吞分选; 非生物逆境胁迫应答调控; 细胞分裂; 病毒复制	2020
	VPS23B (AT5G13860)	病毒复制	2009
ESCRT-I	VPS28A (AT4G21560)	胚胎发育; 生长素响应; 膜蛋白内吞分选; 液泡发育	2020
	VPS28B (AT4G05000)	胚胎发育; 生长素响应; 膜蛋白内吞分选; 液泡发育; 植物免疫	2020
	VPS37-1 (AT3G53120)	植物免疫	2013
	VPS37-2 (AT2G36680)
	VPS22 (AT4G27040)	水稻主根生长; 胚乳灌浆	2013
ESCRT-II	VPS25 (AT4G19003)	生长素响应; 主根生长	2014
ESCRT-II	VPS36 (AT5G04920)	胚胎发育; 主根生长; MVB/PVC发育; 液泡发育; 膜蛋白内吞分选	2017
	VPS2.1 (AT2G06530)	胚胎发育; MVB/PVC发育; 膜蛋白内吞分选; 自噬途径	2020
	VPS2.2 (AT5G44560)
	VPS2.3 (AT1G03950)
	VPS20.1 (AT5G63880)	MVB/PVC发育; 膜蛋白内吞分选; 病毒复制	2014
ESCRT-III	VPS20.2 (AT5G09260)
	SNF7A (AT2G19830)	MVB/PVC发育; 膜蛋白内吞分选; 病毒复制	2014
	SNF7B (AT4G29160)	MVB/PVC发育; 植物发育; 病毒复制	2021
	VPS24-1 (AT5G22950)	MVB/PVC发育; 膜蛋白内吞分选; 病毒复制	2019
	VPS24-2 (AT3G45000)
	CHMP1A (AT1G73030)	胚胎发育; MVB/PVC发育; 膜蛋白内吞分选, 自噬途径	2015
ESCRT-III辅助蛋白	CHMP1B (AT1G17730)	胚胎发育; MVB/PVC发育; 膜蛋白内吞分选; 自噬途径	2015
	VPS60-1 (AT3G10640)
	VPS60-2 (AT5G04850)
	CHMP7 (AT3G62080)	衰老; 非生物逆境胁迫应答调控	2016
	ISTL (AT1G34220)	MVB/PVC发育; 植物发育; 植物免疫	2016
VPS4/SKD1复合体	VPS4/SKD1 (AT2G27600)	MVB/PVC发育; 膜蛋白内吞分选; 病毒复制; 植物免疫	2016
VPS4/SKD1复合体	LIP5 (AT4G26750)	MVB/PVC发育; 植物发育; 膜蛋白内吞分选; 植物免疫; 非生物逆境胁迫应答调控	2016
	FREE1 (AT1G20110)	MVB/PVC发育; 液泡发育; 膜蛋白内吞分选; 自噬途径; 非生物逆境胁迫应答调控	2020
植物特有蛋白	FYVE4 (AT1G61690)	MVB/PVC发育; 膜蛋白内吞分选	2021
	PROS (AT4G24370)	VPS4/SKD1复合体活性调控; 细胞延伸	2014
	BRAF (AT5G14020)	MVB/PVC发育; 与FREE1竞争性结合VPS23A	2018
	RST1 (AT3G27670)	胚胎发育; 茎角质层蜡的分布; 植物免疫; MVB/ PVC发育	2019

复合体	基因	功能	参考文献
ESCRT-0	N.I.
	VPS23A (AT3G12400)	膜蛋白内吞分选; 非生物逆境胁迫应答调控; 细胞分裂; 病毒复制	2020
	VPS23B (AT5G13860)	病毒复制	2009
ESCRT-I	VPS28A (AT4G21560)	胚胎发育; 生长素响应; 膜蛋白内吞分选; 液泡发育	2020
	VPS28B (AT4G05000)	胚胎发育; 生长素响应; 膜蛋白内吞分选; 液泡发育; 植物免疫	2020
	VPS37-1 (AT3G53120)	植物免疫	2013
	VPS37-2 (AT2G36680)
	VPS22 (AT4G27040)	水稻主根生长; 胚乳灌浆	2013
ESCRT-II	VPS25 (AT4G19003)	生长素响应; 主根生长	2014
ESCRT-II	VPS36 (AT5G04920)	胚胎发育; 主根生长; MVB/PVC发育; 液泡发育; 膜蛋白内吞分选	2017
	VPS2.1 (AT2G06530)	胚胎发育; MVB/PVC发育; 膜蛋白内吞分选; 自噬途径	2020
	VPS2.2 (AT5G44560)
	VPS2.3 (AT1G03950)
	VPS20.1 (AT5G63880)	MVB/PVC发育; 膜蛋白内吞分选; 病毒复制	2014
ESCRT-III	VPS20.2 (AT5G09260)
	SNF7A (AT2G19830)	MVB/PVC发育; 膜蛋白内吞分选; 病毒复制	2014
	SNF7B (AT4G29160)	MVB/PVC发育; 植物发育; 病毒复制	2021
	VPS24-1 (AT5G22950)	MVB/PVC发育; 膜蛋白内吞分选; 病毒复制	2019
	VPS24-2 (AT3G45000)
	CHMP1A (AT1G73030)	胚胎发育; MVB/PVC发育; 膜蛋白内吞分选, 自噬途径	2015
ESCRT-III辅助蛋白	CHMP1B (AT1G17730)	胚胎发育; MVB/PVC发育; 膜蛋白内吞分选; 自噬途径	2015
	VPS60-1 (AT3G10640)
	VPS60-2 (AT5G04850)
	CHMP7 (AT3G62080)	衰老; 非生物逆境胁迫应答调控	2016
	ISTL (AT1G34220)	MVB/PVC发育; 植物发育; 植物免疫	2016
VPS4/SKD1复合体	VPS4/SKD1 (AT2G27600)	MVB/PVC发育; 膜蛋白内吞分选; 病毒复制; 植物免疫	2016
VPS4/SKD1复合体	LIP5 (AT4G26750)	MVB/PVC发育; 植物发育; 膜蛋白内吞分选; 植物免疫; 非生物逆境胁迫应答调控	2016
	FREE1 (AT1G20110)	MVB/PVC发育; 液泡发育; 膜蛋白内吞分选; 自噬途径; 非生物逆境胁迫应答调控	2020
植物特有蛋白	FYVE4 (AT1G61690)	MVB/PVC发育; 膜蛋白内吞分选	2021
	PROS (AT4G24370)	VPS4/SKD1复合体活性调控; 细胞延伸	2014
	BRAF (AT5G14020)	MVB/PVC发育; 与FREE1竞争性结合VPS23A	2018
	RST1 (AT3G27670)	胚胎发育; 茎角质层蜡的分布; 植物免疫; MVB/ PVC发育	2019