Chinese Bulletin of Botany ›› 2022, Vol. 57 ›› Issue (5): 673-683.DOI: 10.11983/CBB22028

• SPECIAL TOPICS • Previous Articles     Next Articles

Recent Advances of Ribosome Profiling in Plants

Wang Yuying, Wang Weihao*()   

  1. Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
  • Received:2022-02-14 Accepted:2022-06-28 Online:2022-09-01 Published:2022-09-09
  • Contact: Wang Weihao
  • About author:*E-mail:

Abstract: With the great development and progress of high-throughput sequencing (HTS) technologies, many newborn techniques have provided solutions to unresolved biological puzzles. Ribosome profiling has emerged as an HTS-based technique that facilitates the genome-wide investigation of translation at single-nucleotide resolution, and it resolves the missing link between transcriptome and translatome. Ribosome profiling not only identifies the mRNAs in translation, but also provides precise positions of the ribosomes on mRNA to help identify the coding regions of the mRNAs. In addition, combined with the parallel RNA sequencing, ribosome profiling can determine the translation efficiency for each mRNA by comparing the rates of protein synthesis and the abundance of mRNAs. Based on its extraordinary advances, ribosome profiling has been successfully applied in animals, plants and microbes, and has thoroughly enhanced our understanding of translational regulation. However, due to the restrictions caused by the physical characteristics of plant cells and tissues, there are still considerable limitations in the application of ribosome profiling in plants. Here, we introduce the experimental principle of ribosome profiling and review the recent progresses made in botanical researches.

Key words: ribosome profiling, translational regulation, upstream open reading frame