Chinese Bulletin of Botany ›› 2021, Vol. 56 ›› Issue (5): 594-604.DOI: 10.11983/CBB21052

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

The Measurement Principles, Methods and Applications of P515

Chunyan Zhang1,2,*(), Xiaojie Pang2   

  1. 1Plant Science Facility of Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
    2Key Laboratory of Photobiology, Chinese Academy of Sciences, Beijing 100093, China
  • Received:2021-03-23 Accepted:2021-06-18 Online:2021-09-01 Published:2021-08-31
  • Contact: Chunyan Zhang

Abstract: The spectral techniques have been widely used in the field of photosynthesis research, such as the light absorption signals P515 and P700 redox kinetics, and chlorophyll fluorescence, which can detect the photosynthetic activities of plants quickly and accurately. P515 signal is widely present in higher plants and algae, which is caused by the shift of absorption spectrum of pigments on thylakoid membrane. We can detect the ratio of PSI to PSII reaction center, the proton conductivity of chloroplast ATP synthase, the cyclic electron flow rate around PSI, the proton motive force (pmf) and its components by the P515 fast and slow kinetics, and study the photoprotective mechanism by simultaneous detection of P515 signal and chlorophyll fluorescence. In this paper, we summarize the main measurement methods of P515, expound its principles, and the applications. The aim is to provide technical supports for further study on the mechanism of photosynthesis.

Key words: P515, ATP synthase, cyclic electron transport, proton motive force