Chinese Bulletin of Botany ›› 2019, Vol. 54 ›› Issue (3): 350-359.DOI: 10.11983/CBB18205

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Use of DNA Barcoding in Identifying Tropical Trees from Dipterocarpaceae

Jianlin Hu1,2,Zhifang Liu1,2,Xiuqin Ci1,Jie Li1,*   

  1. 1. Laboratory of Plant Phylogenetics and Conservation, Center for Integrative Conservation, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China
    2. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2018-09-27 Accepted:2019-02-11 Online:2019-07-01 Published:2019-11-24
  • Contact: Jie Li

Abstract: Plants from Dipterocarpaceae are vital tropical timber trees in Southeast Asia. They have high potential in forest supervision, forest resource protection and other aspects to evaluate the efficiency of DNA barcodes. We obtained 899 sequences from 244 species for 14 genera combined with sequences from the GenBank. We amplified and sequenced four DNA regions (rbcL, matK, trnL-trnF and ITS2) in samples from the family Dipterocarpaceae. The discrimination ability of DNA barcodes was evaluated by using effective sequence ratios, characteristics of sequences, divergence of intra- and inter-varieties, and identification success rates (Best Match, Best Close Match, BLAST and neighbor-joining methods). The ITS2 fragment showed the highest identification efficiency. High-quality ITS2 sequence can be obtained from the leaves of Dipterocarpaceae plants by using an optimized amplification system. The matK fragment showed obvious genetic variation and 100% amplification and sequencing efficiency, and its identification ability was higher than with other chloroplast markers. Therefore, ITS2 and matK are suitable markers for tropical trees from Dipterocarpaceae.

Key words: Dipterocarpaceae, DNA barcoding, ITS2, matK, tropical region