Chinese Bulletin of Botany ›› 2016, Vol. 51 ›› Issue (4): 533-541.DOI: 10.11983/CBB15087

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Introduction of Exogenous DNA of Populus alba into Sect. Aigeiros via Pollen-tube Pathway

Xinwen Zhao*   

  1. Research Institute of Poplar of Liaoning Province, Gaizhou 115200, China
  • Received:2015-05-17 Accepted:2015-09-04 Online:2016-07-01 Published:2016-08-05
  • Contact: Zhao Xinwen
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    # Co-first authors

Abstract:

Sect. Aigeiros is fast-growing and practical and has strong agamogenesis ability. Sect. Leuce has good quality and strong resistance. Many economic traits between them are complementary, so Sect. Aigeiros and Leuce hybrid advantages in polymerization can be obtained from a superior hybrid. However, there are natural incompatibility barriers between Sect. Aigeiros and Leuce. In this study, we introduced the genotype of P. alba into P. × Liaoninggensis sp. nov × P. deltoids cv. ‘N001’ by the pollen-tube pathway to overcome this difficulty. Four of the offspring were similar to P. alba by analysis of phenotype characteristics. From the molecular identification of AFLP, the 4 variant plants were determined to be positive transgenic plants from the genotype of P. alba. Meanwhile, the transformation system of the pollen-tube pathway for Sect. Aigeiros was optimized: the optimal treatment time was 30-72 h, optimal treatment method the uncut stigma method, and optimal treatment 200 μg·mL-1.