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植物学报 >

2024 , Vol. 59 >Issue 5: 738 - 751

DOI: https://doi.org/10.11983/CBB24065

研究报告

水稻抗氧化性状遗传位点挖掘及候选基因分析

  • 连锦瑾 ,
  • 唐璐瑶 ,
  • 张伊诺 ,
  • 郑佳兴 ,
  • 朱超宇 ,
  • 叶语涵 ,
  • 王跃星 ,
  • 商文楠 ,
  • 傅正浩 ,
  • 徐昕璇 ,
  • 吴日成 ,
  • 路梅 ,
  • 王长春 ,
  • 饶玉春
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  • 1浙江师范大学生命科学学院, 金华 321004
    2中国水稻研究所水稻生物育种全国重点实验室, 杭州 310006
    3五常市商博士米业有限公司, 哈尔滨 150200
第一联系人： 共同第一作者
*E-mail: wcc@zjnu.cn;
ryc@zjnu.cn

收稿日期: 2024-04-30

  录用日期: 2024-06-21

  网络出版日期: 2024-06-24

基金资助

浙江省自然科学基金重点项目(LZ23C130003);2024年国家级大学生创新创业训练计划项目和2024年浙江省大学生科技创新活动计划暨新苗人才计划

收起

Genetic Locus Mining and Candidate Gene Analysis of Antioxidant Traits in Rice

  • Jinjin Lian ,
  • Luyao Tang ,
  • Yinuo Zhang ,
  • Jiaxing Zheng ,
  • Chaoyu Zhu ,
  • Yuhan Ye ,
  • Yuexing Wang ,
  • Wennan Shang ,
  • Zhenghao Fu ,
  • Xinxuan Xu ,
  • Richeng Wu ,
  • Mei Lu ,
  • Changchun Wang ,
  • Yuchun Rao
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  • 1College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China
    2State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
    3Wuchang Dr. Shang Rice Industry Co., Ltd., Harbin 150200, China
First author contact: These authors contributed equally to this paper

Received date: 2024-04-30

  Accepted date: 2024-06-21

  Online published: 2024-06-24

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摘要

水稻(Oryza sativa)是世界上最重要的粮食作物之一。提高水稻抗氧化性, 进而提高抗逆性是保障其高产稳产的重要途径。选用籼稻华占(HZ)和粳稻热研2号(Nekken2)及以其为亲本构建的120个重组自交系(RILs), 分别在分蘖期、灌浆期和成熟期测定亲本及其后代剑叶、颖壳及籽粒中羟基自由基清除率、总酚含量、黄酮含量和花青苷含量, 同时基于已构建的高密度遗传连锁图谱进行数量性状基因座(QTL)定位。结果共挖掘到62个与水稻抗氧化损伤有关的QTLs, 其中LOD值最高达4.36。对这些QTL区间内相关候选基因的表达进行定量分析, 结果表明LOC_Os06g01850、LOC_Os12g07820、LOC_ Os12g07830和LOC_Os03g60509等13个基因的表达在不同时期的双亲间差异显著。研究挖掘到众多与水稻抗氧化性相关的QTLs, 为进一步定位并克隆相关基因, 选育抗性强且营养价值高的水稻新品种奠定基础。

关键词： 水稻; 抗氧化性; 遗传图谱; QTL定位

本文引用格式

连锦瑾 , 唐璐瑶 , 张伊诺 , 郑佳兴 , 朱超宇 , 叶语涵 , 王跃星 , 商文楠 , 傅正浩 , 徐昕璇 , 吴日成 , 路梅 , 王长春 , 饶玉春 . 水稻抗氧化性状遗传位点挖掘及候选基因分析[J]. 植物学报, 2024 , 59(5) : 738 -751 . DOI: 10.11983/CBB24065

Abstract

Rice (Oryza sativa) is one of the most important food crops in the world. Improving its antioxidant ability and stress resistance is an important way to ensure high and stable yields. In this study, we used the indica rice HZ and the japonica rice Nekken2 as parents and the 120 recombinant inbred line population constructed from them as experimental materials to determine the hydroxyl radical scavenging rate, total phenol content, flavonoid content, and anthocyanin content in sword leaves, glume shells and grains of parents and their progeny at three stages: the tillering stage, the grain filling stage and the maturity stage. Additionally, a total of 62 QTLs related to rice antioxidant damage were identified on the basis of the constructed high-density genetic map for QTL mapping, with an LOD value of up to 4.36. A quantitative analysis of candidate genes related to antioxidant damage ability in these regions revealed that thirteen candidate genes, including LOC_Os06g01850, LOC_Os12g07820, LOC_Os12g07830, and LOC_Os03g60509 were significantly differentially expressed between the two parents at different growth stages. A multitude of QTLs associated with antioxidant damage resistance in rice were identified, providing a foundation for further mapping and cloning of related genes and the development of new rice varieties with increased resistance and nutritional value.

Key words： rice; antioxidant ability; genetic map; QTL mapping

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