成熟禾谷类种子胚乳细胞的显微CT扫描方法

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  • 1中国科学院植物研究所, 系统与进化植物学国家重点实验室, 北京 100093; 2中国农业科学院作物科学研究所,北京 100081;3贵州省农业科学院,贵州省亚热带作物研究所,贵州兴义562400

收稿日期: 2024-02-18

  修回日期: 2024-03-22

  网络出版日期: 2024-04-02

Micro CT Scanning Method for Endosperm Cells of Mature Cereal Seeds

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  • 1State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; 2Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China3Guizhou Institute of Subtropical Crops, Guizhou Academy of Agricultural Sciences, Xingyi 562400, China

Received date: 2024-02-18

  Revised date: 2024-03-22

  Online published: 2024-04-02

摘要

禾谷类种子淀粉胚乳是人类主食的重要来源,但研究其成熟细胞形态的方法非常有限。显微 CT 技术是一种非破坏性的三维成像技术,是研究植物形态的有力工具。但由于成熟禾谷类种子淀粉胚乳密度均一,常规显微CT技术无法应用。本文在之前研究的基础上,选择十种不同的禾谷类种子,使用磷钨酸长时间处理,经CO2临界点干燥后再用显微CT扫描,可以清晰地分辨出禾谷类淀粉胚乳的细胞形态,为研究此类细胞结构提供了一种新方法。

本文引用格式

徐秀苹, 杨小雨, 冯旻 . 成熟禾谷类种子胚乳细胞的显微CT扫描方法[J]. 植物学报, 0 : 0 -0 . DOI: 10.11983/CBB24022

Abstract

Cereal starch endosperm is an important source of human staple food, but methods for observing its mature cell structure are very limited. Micro CT technology,a non-destructive three-dimensional imaging technique,is a powerful tool for studying plant morphology. However, due to the uniform density of cereal starch endosperm, conventional micro CT techniques cannot be applied for this case. In this article ten kinds of different cereal seeds were treated by phosphotungstic acid, and dried by CO2 critical point dryer, according to author’s previous research; then the cell structure of cereal starch endosperm can be clearly distinguished by Micro CT. This paper provides a new method for micro CT scanning of cereal starch endosperm cells.
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