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植物学报 >

2025 , Vol. 60 >Issue 1: 81 - 89

DOI: https://doi.org/10.11983/CBB24022

技术方法

成熟禾谷类作物种子胚乳细胞的显微CT扫描方法

  • 徐秀苹 ,
  • 杨小雨 ,
  • 冯旻
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  • 1中国科学院植物研究所, 系统与进化植物学重点实验室, 北京 100093
    2国家植物园, 北京 100093
    3中国农业科学院作物科学研究所, 北京 100081
    4贵州省农业科学院, 贵州省亚热带作物研究所, 兴义 562400
徐秀苹, 中国科学院植物研究所正高级工程师, 植物形态与结构平台主管, 负责显微CT扫描系统和冷冻扫描电镜等大型仪器的运行、管理及功能开发。第一完成人授权发明专利3项、实用新型专利1项。以第一作者发表技术方法类文章4篇, 出版仪器图书1册。完成2项中国科学院仪器设备功能开发项目。入选2022年度中国科学院技术支撑人才项目

收稿日期: 2024-02-18

  录用日期: 2024-03-30

  网络出版日期: 2024-04-02

基金资助

中国科学院技术支撑人才项目(2022);黔农科院青年科技基金(2021-20);黔农科院国基后补助(2021-12)

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A New Cereal Seed Treatment Method for Displaying Endosperm Cell Structures Under Micro CT Scanning

  • Xiuping Xu ,
  • Xiaoyu Yang ,
  • Min Feng
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  • 1Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
    2China National Botanical Garden, Beijing 100093, China
    3Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    4Guizhou Institute of Subtropical Crops, Guizhou Academy of Agricultural Sciences, Xingyi 562400, China

Received date: 2024-02-18

  Accepted date: 2024-03-30

  Online published: 2024-04-02

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摘要

禾谷类种子淀粉胚乳是人类主食的重要来源, 但观察其成熟细胞形态的方法非常有限。显微CT是一种非破坏性的三维成像技术, 是研究植物形态的有力工具。由于成熟禾谷类种子淀粉胚乳密度均匀, 常规显微CT技术无法应用。在前期研究的基础上, 用磷钨酸处理10种不同禾谷类作物种子, 经CO2临界点干燥后, 再用显微CT扫描, 可以清晰地分辨出禾谷类作物淀粉胚乳的细胞结构, 为研究此类细胞结构提供了一种新方法。

关键词： 显微CT; 禾谷类淀粉胚乳; 磷钨酸

本文引用格式

徐秀苹 , 杨小雨 , 冯旻 . 成熟禾谷类作物种子胚乳细胞的显微CT扫描方法[J]. 植物学报, 2025 , 60(1) : 81 -89 . DOI: 10.11983/CBB24022

Abstract

Cereal starch endosperm is the main source of human staple food, but the methods for observing its mature cell structure are not well developed. Micro CT technology, a non-destructive three-dimensional imaging technique, is a powerful tool for studying plant morphology. However, due to the uniform density of cereal starch endosperm, whose cell structures cannot be distinguished clearly by conventional micro CT techniques. In this study, we used phosphotungstic acid to treat ten different kinds of cereal seeds of seven crops, and then dried them by CO2 critical point dryer. We found that the cell structure of the treated endosperms can be clearly displayed by micro CT. Our method provides a new way for studying the structures and functions of cereal starch endosperm cells.

Key words： micro CT; cereal starch endosperm; phosphotungstic acid

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参考文献

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