伊藤杂种‘和谐’组培快繁体系的建立
- 1湖南农业大学园艺学院, 长沙 410128
2湖南农业大学风景园林与艺术设计学院, 长沙 410128
3湖南农业大学湖南省中亚热带优质花木繁育与利用工程技术研究中心, 长沙 410128
4中国科学院植物研究所植物多样性与特色经济作物全国重点实验室, 北京 100093
5国家植物园, 北京 100093
6天香源(辽宁)生物科技有限责任公司, 葫芦岛 125300
彭丽平, 中国科学院植物研究所副研究员。长期从事牡丹群体遗传、新品种培育、组织快繁以及有效成分功能评价等研究。以通讯作者和第一作者身份在Horticulture Research、Physiologia Plantarum和Foods等国际主流学术期刊上发表研究论文10余篇。pengliping@ibcas.ac.cn
收稿日期: 2023-08-03
录用日期: 2023-12-19
网络出版日期: 2023-12-29
基金资助
国家自然科学基金青年科学基金(32201609);中国科学院植物研究所企业横向项目(E0119C6001)
Establishment of a Fast Breeding System for Itoh Hybrid ‘He Xie’ in Tissue Culture
- Min Kang ,
- Meiying Zhang ,
- Xiushuang Qi ,
- Ningning Tong ,
- Yang Li ,
- Qingyan Shu ,
- Zheng’an Liu ,
- Changping Lü ,
- Liping Peng
- 1College of Horticulture, Hunan Agricultural University, Changsha 410128, China
2College of Landscape Architecture and Art Design, Hunan Agricultural University, Changsha 410128, China
3Hunan Provincial Engineering and Technology Research Center for Breeding and Utilization of Central-subtropical High-quality Flowering Trees, Hunan Agricultural University, Changsha 410128, China
4National Key Laboratory of Plant Diversity and Special Economic Crops, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
5National Botanical Garden, Beijing 100093, China
6Tian Xiang Yuan (Liaoning) Biotechnology Co. Ltd., Huludao 125300, China
Received date: 2023-08-03
Accepted date: 2023-12-19
Online published: 2023-12-29
摘要
以伊藤杂种‘和谐’(Itoh hybrids ‘He Xie’)的鳞芽为材料建立离体快繁技术体系, 可克服传统方法繁育较慢的缺点, 加速伊藤杂种优良品种的繁育推广。采用单因子试验设计, 分别探究不同灭菌时间、不同植物生长调节剂浓度、不同根诱导时间以及不同生根苗等级对‘和谐’组培苗启动、增殖、生根和驯化效果的影响。结果表明, 用2%次氯酸钠溶液的最佳灭菌时间为12分钟, 鳞芽污染率为9.09%; 最佳初始培养基配方为MS+1.5 mg∙L-1 6-BA+0.2 mg∙L-1 GA3+0.5 mg∙L-1 AgNO3; 最佳增殖培养基配方为MS+450 mg∙L-1 CaCl2+0.5 mg∙L-1 6-BA+0.2 mg∙L-1 IBA+0.2 mg∙L-1 GA3+0.5 mg∙L-1 AgNO3, 增殖系数为3.3; 无根苗在根诱导培养基1/2MS+1.0 mg∙L-1腐胺+2.0 mg∙L-1 IBA上, 经4°C低温暗培养8天后常温光照培养30天, 再转入根形成培养基1/2MS+1.0 g∙L-1 AC, 培养20天生根率达66.7%; 苗移栽的基质为珍珠岩:蛭石:草炭土=1:1:1 (v/v/v), 移栽60天后, 一级苗成活率最高为52.0%, 二级苗和三级苗则大部分死亡, 表明生根质量对移栽成活至关重要。
本文引用格式
康敏 , 张美莹 , 齐秀双 , 佟宁宁 , 李旸 , 舒庆艳 , 刘政安 , 吕长平 , 彭丽平 . 伊藤杂种‘和谐’组培快繁体系的建立[J]. 植物学报, 2024 , 59(3) : 441 -451 . DOI: 10.11983/CBB23105
Abstract
An in vitro rapid propagation system using scale buds of Itoh hybrids ‘He Xie’ can overcome the shortcomings of the slow traditional breeding methods and promote the adoption of the excellent Itoh hybrid varieties. In this study, we used the buds of ‘He Xie’ as explants to investigate the effects of various factors including disinfection time, plant growth regulators (PGRs) concentration and root induction time, and different rooted seedling grades on the initiation, proliferation, rooting and domestication of ‘He Xie’ with one-way experimental design. Our results showed: the optimal disinfection time of buds by 2% sodium hypochlorite solution was 12 min, with a contamination rate of 9.09%; the optimal initial culture medium was MS+1.5 mg∙L-1 6-BA+0.2 mg∙L-1 GA3+0.5 mg∙L-1 AgNO3; the optimal proliferation culture medium was MS+450 mg∙L-1 CaCl2+0.5 mg∙L-1 6-BA+0.2 mg∙L-1 IBA+0.2 mg∙L-1 GA3+0.5 mg∙L-1 AgNO3 with a proliferation rate of 3.3. Rootless seedlings were cultured on root induction medium 1/2MS+1.0 mg∙L-1 putrescine+2.0 mg∙L-1 IBA for 8 d at 4°C in dark and then 30 d at room temperature under light, and finally transferred to root formation medium 1/2MS+1.0 g∙L-1 AC for 20 d, a rooting rate of 66.7% was observed. The rooted seedlings were transplanted on a growing matrix of perlite:vermiculite:charcoal soil=1:1:1 (v/v/v) for 60 d, with the highest transplant survival rate of 52.0% being observed for first-grade rooted seedlings, but most of the second- and third-grade seedlings being dead, suggesting that the quality of rooting is critical for transplant survival.
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