适配体技术及其在植物科学研究中的应用
收稿日期: 2023-04-14
录用日期: 2023-08-19
网络出版日期: 2023-10-10
基金资助
中央高校基本科研业务费专项资金(BLX202116);中国科学院青年创新促进会(2019081);国家自然科学基金(32030010);国家自然科学基金(32000558);北京市自然科学基金(5232016);抗松材线虫病高产油松新品种设计与培育(2022ZD0401605)
Aptamers and Their Applications in Plant Science Researches
Received date: 2023-04-14
Accepted date: 2023-08-19
Online published: 2023-10-10
适配体是一种从人工合成的文库中筛选出来、能够特异结合靶标分子的单链寡核苷酸或多肽。适配体基于自身结构和序列与靶分子特异结合并调节其活性, 应用于生物体内分子功能研究及新型药物制剂的研发。近年来, 多肽适配体广泛应用于医学、遗传学和分子生物学等多个领域, 成为一种高效、特异且功能强大的新工具。在植物研究中, 随着相应体系的建立、应用和推广, 适配体技术逐渐成为研究植物分子功能的有效工具。该文综述了不同类型的适配体、筛选原理及优缺点, 以及在植物研究中的应用。可以预见, 随着分子设计育种技术的发展, 适配体技术有望成为植物科学领域有价值的应用工具。
李奕, 张曦, 袁艳辉, 公丕昌, 林金星 . 适配体技术及其在植物科学研究中的应用[J]. 植物学报, 2023 , 58(6) : 935 -945 . DOI: 10.11983/CBB23050
Aptamers are single-stranded oligonucleotides or short peptides that are screened and selected from artificially synthesized libraries and can specifically bind to the target molecules. Aptamers can bind and regulate the activities of its target molecules based on their own structure and sequence, and are applied to in vivo molecular function researches and new drug preparation explorations. In recent years, peptide aptamers have been widely used in various fields, such as medicine, genetics and molecular biology, which is becoming a highly efficient, specific and powerful new tool. In plants, with the establishment, application and promotion of the corresponding system, aptamers have gradually been become an effective tool for studying plant molecular functions. In this review, we summarized the classification, screening principles and basic applications of aptamers, with special emphasis on their applications in plant breeding. It can be expected that with the development of molecular design bio-breeding technology, aptamers can be cooperated with transgenic techniques and become a valuable application tool in the plant science fields.
Key words： aptamer; nucleic acid; peptide; plants; screening
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