利用转基因毛状根高效培育大豆嵌合植株的方法
收稿日期: 2023-02-20
录用日期: 2023-05-31
网络出版日期: 2023-05-31
基金资助
国家自然科学基金(32072661)
A Highly Efficient Method to Generate Chimeric Soybean Plant with Transgenic Hairy Roots
Received date: 2023-02-20
Accepted date: 2023-05-31
Online published: 2023-05-31
建立高效的大豆(Glycine max)转基因毛状根嵌合植株体系对于推动大豆功能基因组学研究具有重要意义。该研究利用3种大豆基因型材料比较了不同共培养条件下毛状根诱导率及成活率。结果显示, 用发根农杆菌(Agrobacterium rhizogenes)侵染外植体并在黑暗条件下共培养1天是诱导毛状根形成的有效策略。研究发现清除下胚轴处不定根可显著增加毛状根的数目并促进根系生长, 进而提高转基因毛状根的阳性率。毛状根诱导14天接种根瘤菌, 可增强生长初期转基因毛状根与根瘤菌的接触, 从而提高大豆的结瘤效率。该研究成功建立了一种高效培育大豆转基因毛状根嵌合植株的方法, 可广泛应用于大豆基因功能研究。
陈佳欣 , 梅浩 , 黄彩翔 , 梁宗原 , 全依桐 , 李东鹏 , 布威麦尔耶姆·赛麦提 , 李欣欣 , 廖红 . 利用转基因毛状根高效培育大豆嵌合植株的方法[J]. 植物学报, 2024 , 59(1) : 89 -98 . DOI: 10.11983/CBB23021
Chimeric soybean plants with transgenic hairy roots is very important for soybean functional genomics. In this study, we used three soybean genotypes to compare their hairy root induction rate and plant survival rate under different co-cultivation conditions. Our results showed that co-culturing the explants infected by Agrobacterium rhizogenes for 1 d under dark conditions was an effective strategy to induce hairy roots. We also found that removing the adventitious roots (AR) at hypocotyl significantly increased number of hairy roots, enhanced their growth and subsequently improved the positive rate of transgenic hairy roots. Furthermore, we found that the inoculation with rhizobium at 14 d of induction was able to enhance the contact between the bacteria and the transgenic hairy roots at early growth stages, and thus improved the soybean’s nodulation efficiency. Taken together, we successfully established a simple and efficient method to generate chimeric soybean plants with transgenic hairy roots. This method can be widely used in soybean gene functional studies.
Key words： soybean; hairy root; chimeric plant; nodulation; optimization
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