Bna-miR43介导甘蓝型油菜响应干旱胁迫

张盈川, 吴晓明玉, 陶保龙, 陈丽, 鲁海琴, 赵伦, 文静, 易斌, 涂金星, 傅廷栋, 沈金雄

doi:10.11983/CBB22223

植物学报 >

2023 , Vol. 58 >Issue 5: 701 - 711

DOI: https://doi.org/10.11983/CBB22223

研究报告

Bna-miR43介导甘蓝型油菜响应干旱胁迫

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1.华中农业大学作物遗传改良全国重点实验室/国家油菜工程技术研究中心, 武汉 430070
2.长江师范学院现代农业与生物工程学院, 重庆 408100

收稿日期: 2022-09-16

录用日期: 2023-02-25

网络出版日期: 2023-03-06

基金资助

湖北省重点研发计划(2022ABA001)

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Bna-miR43 Mediates the Response of Drought Tolerance in Brassica napus

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1. National Engineering Research Center of Rapeseed/National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
2. School of Advanced Agriculture and Bioengineering, Yangtze Normal University, Chongqing 408100, China

Received date: 2022-09-16

Accepted date: 2023-02-25

Online published: 2023-03-06

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摘要

miRNA通过调控靶基因的表达参与植物生长发育和响应逆境胁迫等多个方面。对前期鉴定到的1个miRNA——Bna-miR43进行功能研究, 通过构建过表达载体探讨了Bna-miR43在甘蓝型油菜(Brassica napus)响应干旱胁迫中的功能。降解组测序预测到Bna-miR43的4个靶基因均属于F-box蛋白家族。在模拟干旱条件下, 甘蓝型油菜J572根系Bna-miR43的表达量逐渐下降; 靶基因则呈现与Bna-miR43相反的表达模式, 且随着干旱处理时间的增加, 靶基因的表达量逐渐上升。转基因实验表明, 在干旱胁迫下, 油菜Bna-miR43过表达株系表现为对干旱极度敏感, 转基因株系种子在干旱条件下发芽率显著下降, 植株脱水严重, 体内积累了更多的丙二醛(MDA)和过氧化氢(H₂O₂)。干旱处理后, Bna-miR43过表达株系中编码超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和乙醇酸氧化酶(GOX)的3个基因下调表达。上述结果表明, Bna-miR43通过调控靶基因的表达调节甘蓝型油菜渗透物质积累和细胞活性氧(ROS)稳态, 在调控植物耐旱性中发挥重要作用。

关键词： 甘蓝型油菜; Bna-miR43; F-box; 干旱胁迫

本文引用格式

张盈川, 吴晓明玉, 陶保龙, 陈丽, 鲁海琴, 赵伦, 文静, 易斌, 涂金星, 傅廷栋, 沈金雄 . Bna-miR43介导甘蓝型油菜响应干旱胁迫[J]. 植物学报, 2023 , 58(5) : 701 -711 . DOI: 10.11983/CBB22223

Abstract

miRNAs are involved in plant growth and response to stress by regulating the expression of target genes. We conducted a functional study on a single miRNA identified by our group, Bna-miR43. The function of Bna-miR43 under drought stress was investigated by constructing a Bna-miR43 overexpression vector. Degradation group sequencing has predicted that the four target genes of Bna-miR43 belonged to the F-box protein family. Under simulated drought conditions, the expression of Bna-miR43 in J572 roots decreased gradually, while the expression pattern of target genes was shown to be opposite to that of Bna-miR43. With the increaseing time of drought treatment, the expression of target genes increased gradually. Expression pattern analysis showed that Bna-miR43 could respond to drought stress by negatively regulating the expression of target genes. Transgenic experiment showed that under drought stress, the Bna-miR43 overexpressed lines were extremely sensitive to drought. It was shown that the germination rate of the overexpressed lines decreased significantly, the plant dehydration was serious, and more MDA and H₂O₂ were accumulated in the body. After drought treatment, three coding superoxide dismutase (SOD), catalase (CAT) and glycolate oxidase (GOX) were identified in the Bna-miR43 overexpressed lines, and their expressions were down-regulated in the Bna-miR43 overexpressed lines. The results showed that Bna-miR43 plays a key role in regulating plant drought tolerance by regulating the osmotic accumulation and ROS homeostasis in Brassica napus.

Key words： Brassica napus; Bna-miR43; F-box; drought stress

参考文献

[1]	陈丽 (2018). 甘蓝型油菜株型及角果长度相关miRNA和靶基因的挖掘. 博士论文. 武汉: 华中农业大学. pp. 21-59.
[2]	宋凝曦, 谢寅峰, 李霞 (2020). 干旱胁迫下表观遗传机制对转C₄型PEPC基因水稻种子萌发的影响. 植物学报 55, 677-692.
[3]	王劲东, 周豫, 余佳雯, 范晓磊, 张昌泉, 李钱峰, 刘巧泉 (2020). MiR172-AP2模块调控植物生长发育及逆境响应的研究进展. 植物学报 55, 205-215.
[4]	吴丹丹, 陈永坤, 杨宇, 孔春艳, 龚明 (2021). 小桐子半胱氨酸蛋白酶家族和相应miRNAs的鉴定及其对低温锻炼的响应. 植物学报 56, 544-558.
[5]	张盈川, 吴晓明玉, 陶保龙, 陈丽, 鲁海琴, 赵伦, 文静, 易斌, 涂金星, 傅廷栋, 沈金雄 (2023). Bna-miR43-FBXL调控模块参与甘蓝型油菜铝胁迫的功能分析. 作物学报 49, 1211-1221.
[6]	Akram NA, Iqbal M, Muhammad A, Ashraf M, Al-Qurainy F, Shafiq S (2018). Aminolevulinic acid and nitric oxide regulate oxidative defense and secondary metabolisms in canola (Brassica napus L.) under drought stress. Protoplasma 255, 163-174.
[7]	An J, Li QX, Yang JJ, Zhang GQ, Zhao ZX, Wu YZ, Wang Y, Wang W (2019). Wheat F-box protein TaFBA1 positively regulates plant drought tolerance but negatively regulates stomatal closure. Front Plant Sci 10, 1242.
[8]	An JP, Rui L, Qu FJ, You CX, Wang XF, Hao YJ (2016). Apple F-Box protein MdMAX2 regulates plant photomorphogenesis and stress response. Front Plant Sci 7, 1685.
[9]	Bai QQ, Wang XY, Chen X, Shi GQ, Liu ZP, Guo CJ, Xiao K (2018). Wheat miRNA TaemiR408 acts as an essential mediator in plant tolerance to Pi deprivation and salt stress via modulating stress-associated physiological processes. Front Plant Sci 9, 499.
[10]	Baldoni E, Genga A, Cominelli E (2015). Plant MYB transcription factors: their role in drought response mechanisms. Int J Mol Sci 16, 15811-15851.
[11]	Batool T, Ali S, Seleiman MF, Naveed NH, Ali A, Ahmed K, Abid M, Rizwan M, Shahid MR, Alotaibi M, Al-Ashkar I, Mubushar M (2020). Plant growth promoting rhizobacteria alleviates drought stress in potato in response to suppressive oxidative stress and antioxidant enzymes activities. Sci Rep 10, 16975.
[12]	Bu Q, Lv T, Shen H, Luong P, Wang J, Wang Z, Huang Z, Xiao L, Engineer C, Kim TH, Schroeder JI, Huq E (2014). Regulation of drought tolerance by the F-Box protein MAX2 in Arabidopsis. Plant Physiol 164, 424-439.
[13]	Danquah A, De Zelicourt A, Colcombet J, Hirt H (2014). The role of ABA and MAPK signaling pathways in plant abiotic stress responses. Biotechnol Adv 32, 40-52.
[14]	Hu Y, Wang B, Hu TX, Chen H, Li H, Zhang W, Zhong Y, Hu HL (2015). Combined action of an antioxidant defence system and osmolytes on drought tolerance and post- drought recovery of Phoebe zhennan S. Lee saplings. Acta Physiol Plant 37, 84.
[15]	Ji XM, Dong BD, Shiran B, Talbot MJ, Edlington JE, Hughes T, White RG, Gubler F, Dolferus R (2011). Control of abscisic acid catabolism and abscisic acid homeostasis is important for reproductive stage stress tolerance in cereals. Plant Physiol 156, 647-662.
[16]	Kaur G, Asthir B (2017). Molecular responses to drought stress in plants. Biol Plant 61, 201-209.
[17]	Kim H, Lee K, Hwang H, Bhatnagar N, Kim DY, Yoon IS, Byun MO, Kim ST, Jung KH, Kim BG (2014). Overexpression of PYL5 in rice enhances drought tolerance, inhibits growth, and modulates gene expression. J Exp Bot 65, 453-464.
[18]	Li WX, Oono Y, Zhu JH, He XJ, Wu JM, Iida K, Lu XY, Cui XP, Jin HL, Zhu JK (2008). The Arabidopsis NFYA5 transcription factor is regulated transcriptionally and posttranscriptionally to promote drought resistance. Plant Cell 20, 2238-2251.
[19]	Liu HH, Tian X, Li YJ, Wu CA, Zheng CC (2008). Microarray-based analysis of stress-regulated microRNAs in Arabidopsis thaliana. RNA 14, 836-843.
[20]	Liu XY, Zhang XJ, Sun BC, Hao LY, Liu C, Zhang DF, Tang HJ, Li CH, Li YX, Shi YS, Xie XQ, Song YC, Wang TY, Li Y (2019). Genome-wide identification and comparative analysis of drought-related microRNAs in two maize inbred lines with contrasting drought tolerance by deep sequencing. PLoS One 14, e0219176.
[21]	Nadarajah K, Kumar IS (2019). Drought response in rice: the miRNA story. Int J Mol Sci 20, 3766.
[22]	Nadeem M, Li JJ, Yahya M, Sher A, Ma CX, Wang XB, Qiu LJ (2019). Research progress and perspective on drought stress in legumes: a review. Int J Mol Sci 20, 2541.
[23]	Shah SMS, Ullah F (2021). A comprehensive overview of miRNA targeting drought stress resistance in plants. Braz J Biol 83, e242708.
[24]	Wei LY, Zhang DF, Xiang F, Zhang ZX (2009). Differentially expressed miRNAs potentially involved in the regulation of defense mechanism to drought stress in maize seedlings. Int J Plant Sci 170, 979-989.
[25]	Wu JD, Jiang YL, Liang YN, Chen L, Chen WJ, Cheng BJ (2019). Expression of the maize MYB transcription factor ZmMYB3R enhances drought and salt stress tolerance in transgenic plants. Plant Physiol Biochem 137, 179-188.
[26]	Wu MF, Tian Q, Reed JW (2006). Arabidopsis microRNA167 controls patterns of ARF6 and ARF8 expression, and regulates both female and male reproduction. Development 133, 4211-4218.
[27]	Xiong HY, Li JJ, Liu PL, Duan JZ, Zhao Y, Guo X, Li Y, Zhang HL, Ali J, Li ZC (2014). Overexpression of OsMYB48-1, a novel MYB-related transcription factor, enhances drought and salinity tolerance in rice. PLoS One 9, e92913.
[28]	Xu D, Duan X, Wang B, Hong B, Ho THD, Wu R (1996). Expression of a late embryogenesis abundant protein gene, HVA1, from barley confers tolerance to water deficit and salt stress in transgenic rice. Plant Physiol 110, 249-257.
[29]	Zhao ZX, Zhang GQ, Zhou SM, Ren YQ, Wang W (2017). The improvement of salt tolerance in transgenic tobacco by overexpression of wheat F-box gene TaFBA1. Plant Sci 259, 71-85.
[30]	Zhou SM, Sun XD, Yin SH, Kong XZ, Zhou S, Xu Y, Luo Y, Wang W (2014). The role of the F-box gene TaFBA1 from wheat (Triticum aestivum L.) in drought tolerance. Plant Physiol Biochem 84, 213-223.

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