研究报告
2019-2021年我国南方稻区白叶枯病菌的毒力与遗传多样性调查研究
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- 1.浙江师范大学生命科学学院, 金华 321004
2.中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程, 北京 100081
3.苏州市植物保护植物检疫站, 苏州 215006
4.中国农业科学院三亚国家南繁研究院, 三亚 572024
第一联系人：
† 共同第一作者。
收稿日期: 2022-08-03
录用日期: 2023-02-09
网络出版日期: 2023-03-06
基金资助
国家重点研发计划(2021YFD1200501-5);国家自然科学基金(U20A2035);中央级公益性科研院所基本科研业务费专项(Y2022QC001)
Genotypic Diversity and Pathogenisity of Xanthomonas oryzae pv. oryzae Isolated from Southern China in 2019-2021
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- 1. College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China
2. National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Sciences, Chinese Academy of Agriculture Sciences (CAAS), Beijing 100081, China
3. Suzhou Municipal Plant Protection and Quarantine Station, Suzhou 215006, China
4. National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, China
First author contact:
† These authors contributed equally to this paper.
Received date: 2022-08-03
Accepted date: 2023-02-09
Online published: 2023-03-06
摘要
种植抗病品种一直是防控水稻白叶枯病(bacterial blight, BB)最有效的措施。近年来, 白叶枯病在我国多地呈现“老病新发”态势。为查明近期白叶枯病成灾的原因, 2019-2021年间, 在南方8省(海南、云南、广西、广东、福建、湖南、浙江和江苏)病害重发生田块采集叶片, 分离获得野生白叶枯病菌(Xanthomonas oryzae pv. oryzae, Xoo)。通过对主效毒性因子基因型tale (transcription activator-like effectors)进行Southern杂交检测, 将新分离的97株Xoo菌株划分为10个基因型, 其中基因型V是优势种群代表。选取各基因型的代表菌株, 剪叶接种携带主要抗病基因(R gene)的水稻(Oryza sativa)品种, 毒力测试结果显示, Xa3和Xa4等传统抗病基因对田间大部分菌株已经丧失抗性, Xa7和Xa23等优异抗病基因对白叶枯病仍具有广谱抗性。研究结果表明, 近期“老病新发”的主要原因可能是水稻新品种选育过程中忽视了优异抗白叶枯病基因资源的引入, 挖掘与利用优异抗病基因资源仍是防控白叶枯病最理想的途径。
本文引用格式
田传玉, 方妍力, 沈晴, 王宏杰, 陈析丰, 郭威, 赵开军, 王春连, 纪志远 . 2019-2021年我国南方稻区白叶枯病菌的毒力与遗传多样性调查研究[J]. 植物学报, 2023 , 58(5) : 743 -749 . DOI: 10.11983/CBB22183
Abstract
Although resistant cultivars have been widely employed as the most economical and effective way to control bacterial blight (BB) in rice, the outbreak of BB reoccured in Southern China in recent years. In order to investigate the cause for the recent outbreak of BB in China, we collected the rice leaves with typical BB symptom from paddies in eight southern provinces (Hainan, Yunnan, Guangxi, Guangdong, Fujian, Hunan, Zhejiang, and Jiangsu) in 2019 to 2021. A total of 97 Xanthomonas oryzae pv. oryzae (Xoo) strains isolated from the BB-diseased leaves were divided into 10 genotypes by Southern hybridization detection of transcription activator-like effectors (tale), of which genotype V was the representative of the dominant population. The representative strains of each genotype were applied to inoculated rice accessions with different resistance (R) gene. The inoculation results revealed that traditional major R genes such as Xa3 and Xa4 were only effective against a small number of the newly isolated Xoo strains, whereas Xa7 and Xa23 still had broad-spectrum resistance to all (or the majority of) these strains. We concluded that the cause for the recent outbreak of rice BB in Southern China is most likely the insufficient use of effective BB resistance genes in the new rice varieties. Exploring and utilizing excellent resistance gene resources is still the most ideal way to prevent and control BB.
参考文献
| [1] | 张华, 姜英华, 胡白石, 刘凤权, 许志刚 (2008). 利用PCR技术专化性检测水稻细菌性条斑病菌. 植物病理学报 38, 1-5. |
| [2] | Antony G, Zhou JH, Huang S, Li T, Liu B, White F, Yang B (2010). Rice xa13 recessive resistance to bacterial blight is defeated by induction of the disease susceptibility gene Os-11N3. Plant Cell 22, 3864-3876. |
| [3] | Boch J, Bonas U, Lahaye T (2014). TAL effectors-pathogen strategies and plant resistance engineering. New Phy- tol 204, 823-832. |
| [4] | Breia R, Conde A, Badim H, Fortes AM, Gerós H, Granell A (2021). Plant SWEETs: from sugar transport to plant-pathogen interaction and more unexpected physiological roles. Plant Physiol 186, 836-852. |
| [5] | Doyle EL, Stoddard BL, Voytas DF, Bogdanove AJ (2013). TAL effectors: highly adaptable phytobacterial virulence factors and readily engineered DNA-targeting proteins. Trends Cell Biol 23, 390-398. |
| [6] | Ji CH, Ji ZY, Liu B, Cheng H, Liu H, Liu SZ, Yang B, Chen GY (2020). Xa1 Allelic R genes activate rice blight resistance suppressed by interfering TAL effectors. Plant Commun 1, 100087. |
| [7] | Ji ZY, Guo W, Chen XF, Wang CL, Zhao KJ (2022). Plant executor genes. Int J Mol Sci 23, 1524. |
| [8] | Ji ZY, Ji CH, Liu B, Zou LF, Chen GY, Yang B (2016). Interfering TAL effectors of Xanthomonas oryzae neutralize R-gene-mediated plant disease resistance. Nat Commun 7, 13435. |
| [9] | Ji ZY, Wang CL, Zhao KJ (2018). Rice routes of countering Xanthomonas oryzae. Int J Mol Sci 19, 3008. |
| [10] | Ji ZY, Zakria M, Zou LF, Xiong L, Li Z, Ji GH, Chen GY (2014). Genetic diversity of transcriptional activator-like effector genes in Chinese isolates of Xanthomonas oryzae pv. oryzicola. Phytopathology 104, 672-682. |
| [11] | Ni?o-Liu DO, Ronald PC, Bogdanove AJ (2006). Xanthomonas oryzae pathovars: model pathogens of a model crop. Mol Plant Pathol 7, 303-324. |
| [12] | Nowack MK, Holmes DR, Lahaye T (2022). TALE-induced cell death executors: an origin outside immunity? Trends Plant Sci 27, 536-548. |
| [13] | Oliva R, Ji CH, Atienza-Grande G, Huguet-Tapia JC, Perez-Quintero A, Li T, Eom JS, Li CH, Nguyen H, Liu B, Auguy F, Sciallano C, Luu VT, Dossa GS, Cunnac S, Schmidt SM, Slamet-Loedin IH, Vera Cruz C, Szurek B, Frommer WB, White FF, Yang B (2019). Broad- spectrum resistance to bacterial blight in rice using genome editing. Nat Biotechnol 37, 1344-1350. |
| [14] | Perez-Quintero AL, Szurek B (2019). A decade decoded: spies and hackers in the history of TAL effectors research. Annu Rev Phytopathol 57, 459-481. |
| [15] | Sambrook J, Fritsch EF, Maniatis T (1989). Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor: Cold Spring Harbor Laboratory. |
| [16] | Wang CL, Qin TF, Yu HM, Zhang XP, Che JY, Gao Y, Zheng CK, Yang B, Zhao KJ (2014). The broad bacterial blight resistance of rice line CBB23 is triggered by a novel transcription activator-like (TAL) effector of Xanthomonas oryzae pv. oryzae. Mol Plant Pathol 15, 333-341. |
| [17] | White FF, Yang B (2009). Host and pathogen factors controlling the rice-Xanthomonas oryzae interaction. Plant Physiol 150, 1677-1686. |
| [18] | Xu ZY, Xu XM, Gong Q, Li ZY, Li Y, Wang S, Yang YY, Ma WX, Liu LY, Zhu B, Zou LF, Chen GY (2019). Engineering broad-spectrum bacterial blight resistance by simultaneously disrupting variable TALE-binding elements of multiple susceptibility genes in rice. Mol Plant 12, 1434-1446. |
| [19] | Xu ZY, Zou LF, Ma WX, Cai LL, Yang YY, Chen GY (2017). Action modes of transcription activator-like effectors (TALEs) of Xanthomonas in plants. J Integr Agric 16, 2736-2745. |
| [20] | Yang B, Sugio A, White FF (2006). Os8N3 is a host disease-susceptibility gene for bacterial blight of rice. Proc Natl Acad Sci USA 103, 10503-10508. |
| [21] | Zhang BM, Zhang HT, Li F, Ouyang YD, Yuan M, Li XH, Xiao JH, Wang SP (2020). Multiple alleles encoding atypical NLRs with unique central tandem repeats in rice confer resistance to Xanthomonas oryzae pv. oryzae. Plant Commun 1, 100088. |
| [22] | Zhou JH, Peng Z, Long JY, Sosso D, Liu B, Eom JS, Huang S, Liu SZ, Vera Cruz C, Frommer WB, White FF, Yang B (2015). Gene targeting by the TAL effector PthXo2 reveals cryptic resistance gene for bacterial blight of rice. Plant J 82, 632-643. |
| [23] | Zhou JM, Zhang YL (2020). Plant immunity: danger perception and signaling. Cell 181, 978-989. |
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