普通菜豆镰孢菌枯萎病抗性种质资源筛选及全基因组关联分析
收稿日期: 2022-07-12
录用日期: 2023-02-09
网络出版日期: 2023-02-10
基金资助
国家重点研发计划(2020YFD1000800);国家重点研发计划(2020YFD1000802-01);国家食用豆产业技术体系(CARS-08)
Screening of Resistance Germplasm Resources and Genome-wide Association Study of Fusarium Wilt in Common Bean
Received date: 2022-07-12
Accepted date: 2023-02-09
Online published: 2023-02-10
普通菜豆镰孢菌枯萎病是严重制约菜豆(Phaseolus vulgaris)产量的主要病害之一。采用下胚轴双孔注射法对601份普通菜豆种质资源进行枯萎病抗性鉴定, 共筛选出4份高抗材料。在此基础上, 基于分布在全基因组上的3 765 456个单核苷酸多态性(SNP)标记, 进行全基因组关联分析, 以P<1×10-5为阈值。结果检测到57个显著关联的SNP位点, 分布于1、2、6、8和11号染色体上; 共获得8个显著关联区域, 其中位于1号染色体上的区域1包含SNP最多(48个), 最显著SNP P值为2.18E-07。在8个显著关联区域中, 共检测到186个基因, 其中157个基因有注释信息, 编码过氧化物酶、抗病蛋白、转录因子和蛋白激酶等。结合KEGG富集分析和序列同源性比对, 鉴定出9个候选基因可能与抗性相关。
李园, 常玉洁, 王兰芬, 王述民, 武晶 . 普通菜豆镰孢菌枯萎病抗性种质资源筛选及全基因组关联分析[J]. 植物学报, 2023 , 58(1) : 51 -61 . DOI: 10.11983/CBB22149
Fusarium wilt is one of the main diseases that seriously restrict the yield of common bean in China. In this study, the hypocotyl double hole injection method was used to identify the resistance of 601 common bean germplasm resources to fusarium wilt, and 4 highly resistance materials were screened out. Based on 3 765 456 single nucleotide polymorphisms (SNPs) distributed on the whole genome, the genome-wide association study was conducted with P<1×10-5 as the threshold. A total of 57 significant SNPs were detected, which are distributed on chromosomes 1, 2, 6, 8, and 11 respectively. A total of 8 significant associated regions were obtained, of which region 1 on chromosome 1 contained up to 48 SNPs and the most significant SNP P value was 2.18E-07. Furthermore, 186 genes were detected in 8 significant association regions, of which 157 were annotated genes, including encoding peroxidase, disease resistance protein, transcription factor and protein kinase genes. Combing KEGG enrichment analysis and homology comparison, 9 candidate genes were identified that might be related to resistance.
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