褪黑素促进镉胁迫下番茄种子萌发的调控机理

蔡淑钰, 刘建新, 王国夫, 吴丽元, 宋江平

doi:10.11983/CBB22202

植物学报 >

2023 , Vol. 58 >Issue 5: 720 - 732

DOI: https://doi.org/10.11983/CBB22202

研究报告

褪黑素促进镉胁迫下番茄种子萌发的调控机理

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绍兴文理学院元培学院, 绍兴 312000

收稿日期: 2022-08-26

录用日期: 2022-12-01

网络出版日期: 2022-12-23

基金资助

国家自然科学基金(31902095)

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Regulatory Mechanism of Melatonin on Tomato Seed Germination Under Cd²⁺ Stress

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Shaoxing University Yuanpei College, Shaoxing 312000, China

Received date: 2022-08-26

Accepted date: 2022-12-01

Online published: 2022-12-23

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摘要

土壤镉(Cd²⁺)污染严重制约设施蔬菜的产量和品质。褪黑素(MT)可以增强植株对多种逆境的抗性, 然而Cd²⁺胁迫下MT调控番茄(Solanum lycopersicum)种子萌发的下游信号尚不清楚。该研究以番茄野生型Alisa Craig种子为材料, 探究不同浓度Cd²⁺处理及外源MT对番茄种子萌发的调控效应。结果表明, 0.5 mmol·L^-1以上的Cd²⁺处理显著抑制番茄种子的萌发和幼苗生长。用0.15 mmol·L^-1 MT浸种可降低种子萌发后地下部和地上部的Cd²⁺含量, 有效缓解Cd²⁺胁迫对番茄种子萌发和幼苗生长的抑制作用。MT上调了番茄胚根中植物螯合肽和转运蛋白基因(PCS、NRAMP1、ABCC3、HMA3与ABCG5)的表达, 促进Cd²⁺的跨膜转运以及液泡隔离。此外, MT减轻了Cd²⁺诱导的氧化损伤, 除褪黑素自身的抗氧化作用外, 也与抗氧化酶(CAT、APX与ALDH)活性的增强相关。进一步分析表明, MT显著下调了Cd²⁺胁迫下脱落酸(ABA)合成基因(NCED1和NCED2)的表达, 并上调了ABA分解基因ABA8ox1的表达, 降低ABA含量, 从而有效调节赤霉素/脱落酸(GA/ABA)的比值, 促进Cd²⁺胁迫下番茄种子的萌发。

关键词： 褪黑素; 镉胁迫; 番茄; 种子萌发

本文引用格式

蔡淑钰, 刘建新, 王国夫, 吴丽元, 宋江平 . 褪黑素促进镉胁迫下番茄种子萌发的调控机理[J]. 植物学报, 2023 , 58(5) : 720 -732 . DOI: 10.11983/CBB22202

Abstract

Soil cadmium (Cd) pollution seriously restricts the yield and quality of facility vegetables. Melatonin (MT) can enhance the resistance to various stresses in plants. However, the downstream signals, which regulated by MT during tomato seed germination under Cd²⁺ stress remains unclear. The effects of Cd²⁺ stress and exogenous MT on seed germination were investigated using tomato (Solanum lycopersicum) wild-type Alisa Craig seeds. The results showed that the germination of tomato seeds and seedling growth were significantly inhibited by Cd²⁺ treatment with more than 0.5 mmol·L^-1. Exogenous MT (0.15 mmol·L^-1) reduced the content of Cd²⁺ in the underground and above-ground tissues of seedlings, effectively alleviating the inhibitory effect of Cd²⁺ stress on tomato seed germination and seedling length. The expression of phytochelatin and transporters-related genes (PCS, NRAMP1, ABCC3, HMA3, and ABCG5) in tomato radicles were significantly increased by MT under Cd²⁺ stress, showing the positive regulation of MT in transmembrane transport and vacuolar sequestration of Cd²⁺. In addition, MT alleviated the oxidative damage induced by Cd²⁺stress, which was related to the enhanced activities of CAT, APX, and ALDH enzymes apart from its own scavenging ability. Furthermore, MT significantly down-regulated the expressions of abscisic acid (ABA) synthesis genes (NCED1 and NCED2) under Cd²⁺ stress, and up-regulated the expression of ABA decomposition gene ABA8ox1, leading to the decrease of ABA content, which effectively regulated the GA/ABA ratio and promoted the germination of tomato seeds under Cd²⁺ stress.

Key words： melatonin; cadmium stress; tomato; seed germination

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