月季红双喜花瓣变色的化学基础及比较转录组分析
收稿日期: 2022-03-12
录用日期: 2022-06-23
网络出版日期: 2022-07-01
基金资助
河南省自然科学基金(212300410360)
The Chemical Composition and Transcriptome Analysis Reveal the Mechanism of Color Formation in Rosa hybrida cv. ‘Double delight’
Received date: 2022-03-12
Accepted date: 2022-06-23
Online published: 2022-07-01
月季(Rosa hybrida)花色丰富, 是世界著名的观赏花卉。月季红双喜因其花瓣的变色特性而在市场上广受欢迎。该研究通过类黄酮和类胡萝卜素靶向代谢并结合转录组分析, 发现红双喜的黄色花瓣呈色主要源于叶绿素、类胡萝卜素以及类黄酮的积累, 红色花瓣呈色主要是花青素苷积累增加且糖苷化的结果。花青素苷合成关键基因CHI、ANS和UFGT, 以及R2R3-MYB家族的AN2-like成员在红色花瓣中的强烈表达是花青素苷积累的分子基础; 类胡萝卜素成分改变及相关基因的表达变化在红双喜花瓣变色过程中也起重要作用, 并且miRNA156可能参与其调控过程。该研究揭示了月季红双喜花瓣变色的分子和化学基础, 研究结果为观赏植物花色分子设计育种提供了重要理论依据。
张和臣, 王慧娟, 李艳敏, 高杰, 袁欣, 王利民, 王校晨, 赵银鸽, 符真珠 . 月季红双喜花瓣变色的化学基础及比较转录组分析[J]. 植物学报, 2022 , 57(5) : 649 -660 . DOI: 10.11983/CBB22049
Rosa species is one of world-famous ornamental flower with very rich colors. Rosa hybrida cv. ‘Double delight’ is very popular in the market due to the color-changing properties of petals. With analysis of the composition of flavonoids and carotenoids, combined with transcriptome analysis, we showed that the red petals were caused by the increasing of glycosylated anthocyanins, and the up-regulation of anthocyanin synthesis genes CHI, ANS and UFGT by R2R3-MYB transcription factor AN2-likes. However, the yellow petals were mainly due to the accumulation of the pigments of chlorophyll, carotenoids and flavonoids. The structural differences of carotenoids and the expression changes of related genes may play an important role in the petals’ discoloration, and miRNA156 might be involved in the regulation. This study revealed a molecular and chemical basis of the petal discoloration in rose Double delight and provided an important basis for the molecule design and breeding of flowers.
Key words： rose; flower color; anthocyanins; carotenoids; molecular regulation
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