单列毛壳菌通过促进秸秆降解并调控激素响应基因表达促进玉米生长
收稿日期: 2021-08-27
修回日期: 2022-01-13
网络出版日期: 2022-01-13
基金资助
河南省教育厅高等学校重点应用研究项目(21A210012);国家自然科学基金(42007005);河南省青年人才托举工程(2022HYTP036)
Chaetomium uniseriatum Promotes Maize Growth by Accelerating Straw Degradation and Regulating the Expression of Hormone Responsive Genes
Received date: 2021-08-27
Revised date: 2022-01-13
Online published: 2022-01-13
为探究生防真菌单列毛壳菌(Chaetomium uniseriatum)对秸秆降解和玉米(Zea mays)生长的影响, 将单列毛壳菌接种到玉米盆栽土壤中, 其它条件不变, 以保证单一变量。于拔节期和抽雄期进行采样, 通过测定土壤有机碳、土壤可溶性碳/氮、微生物量碳/氮以及酶活性, 探究接种单列毛壳菌对土壤生物化学指标的影响。在抽雄期对秸秆降解率、地上部生物量、叶片SPAD值、玉米根系激素含量及根系转录组进行分析, 探究接种单列毛壳菌对秸秆降解和玉米植株生长发育的影响。结果表明, 接种单列毛壳菌后, 土壤养分含量未出现显著性变化, β-葡萄糖苷酶(β-GC)活性显著降低; 抽雄期玉米地上部生物量、叶片SPAD值以及秸秆降解率均显著高于对照组; 玉米根系生长激素(IAA)和玉米素(ZR)含量均显著低于对照组。不同处理下玉米根系转录组分析筛选得到990个差异表达基因(383个基因表达上调, 607个基因表达下调); 对差异基因进行GO富集分析, 得到5个植物激素相关的条目; KEGG富集分析得到1个与植物激素相关的通路(P value<0.05, Q value<0.05)。综上, 单列毛壳菌通过促进秸秆降解以及调控作物根系激素响应基因的表达, 进而促进玉米生长。
李月, 胡德升, 谭金芳, 梅浩, 王祎, 李慧, 李芳, 韩燕来 . 单列毛壳菌通过促进秸秆降解并调控激素响应基因表达促进玉米生长[J]. 植物学报, 2022 , 57(4) : 422 -433 . DOI: 10.11983/CBB21147
To explore the effect of Chaetomium uniseriatum on straw degradation and maize growth, C. uniseriatum was inoculated into pots planted with maize, with other conditions unchanged to ensure a single variable. The soil organic carbon, microbial biomass C/N, dissolved organic C/N content and enzymes activities were measured to assess the response of soil biochemical properties to C. uniseriatum inoculation at jointing and tasseling stage. The degradation rate of straw, aboveground biomass, SPAD value of leaves, root hormones and root transcriptomes were investigated to verify the influence of C. uniseriatum on the growth of maize. The soil nutrients content did not change significantly in inoculated treatments, while the activity of β-glucosidase (β-GC) decreased significantly. At tasseling stage, the aboveground biomass, SPAD value of leaves and degradation rate of straw were significantly enhanced in inoculated treatments as compared with the control. The contents of auxin (IAA) and zeatin (ZR) in maize roots were significantly lower than those in control. Transcriptome analysis of maize roots revealed that there were 990 differentially expressed genes (607 down regulated and 383 up regulated) between the two treatments. Five GO terms associated with regulation of plant hormones were enriched, and one hormone related pathway was enriched significantly (P value<0.05, Q value<0.05) according to KEGG annotation. Our study revealed that C. uniseriatum could improve maize growth by accelerating straw degradation and regulating the expression of hormone response genes in roots.
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