十字花科植物ABI4序列演化与转录激活活性分析
收稿日期: 2021-02-10
录用日期: 2021-08-09
网络出版日期: 2021-08-11
基金资助
国家自然科学基金(31970228);全国大学生创新创业计划(201910718034)
Analysis on the Evolution and Transcription Activation Activity of ABI4 in Brassicaceae
Received date: 2021-02-10
Accepted date: 2021-08-09
Online published: 2021-08-11
转录因子ABI4参与植物激素ABA的绝大多数生物学功能, 它不仅是ABA和GA在植物体内含量平衡的核心调控因子, 同时还连接ABA与植物细胞内多个信号通路。利用拟南芥(Arabidopsis thaliana) ABI4序列在十字花科其它19种植物(除拟南芥外)中检索得到27个同源基因, 通过序列多态性分析、系统发生重建、染色体共线性分析和转录激活活性比较, 探讨了该基因在十字花科植物中的演化历史。结果表明, ABI4蛋白质序列和结构在十字花科植物中具有较高的保守性, 暗示了其功能的重要性; 单独的ABI4蛋白并不具备显著的转录激活活性, 说明其生物学功能的具体分子机制可能相对复杂, 需要进一步探讨。
张文晶, 杨晓萌, 高侃, 魏欣仪, 石雪彤, 王瑞瑄, 武凤霞, 康菊清 . 十字花科植物ABI4序列演化与转录激活活性分析[J]. 植物学报, 2021 , 56(6) : 676 -686 . DOI: 10.11983/CBB21036
ABI4 is an important component of the ABA signal transduction pathway. It not only acts as a key player in ABA and GA antagonism, but also plays important roles in various aspects of ABA crosstalk with other signal chemicals. Genes regulated by ABI4 are involved in diverse processes. In this study, we got 27 homologs from 19 species in the family Brassicaceae using AthABI4 as a query, and explored their evolutionary history based on sequence polymorphism analysis, phylogenetic reconstruction, genomic synteny assay, and transcription activation activity comparison. The result revealed that ABI4 are highly conserved and might be not work as transcriptional activators independently in the Brassicaceae, which implies that the irreplaceable roles of ABI4 in plants and further research was needed to explore the molecular mechanism of its biological function.
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