氮处理对大豆根瘤固氮能力及GmLbs基因表达的影响
- 福建农林大学根系生物学研究中心, 福州 350002
收稿日期: 2021-02-13
录用日期: 2021-05-07
网络出版日期: 2021-05-07
基金资助
国家自然科学基金(32072661)
Effects of Nitrogen Application on Nitrogen Fixation Capacity and GmLbs Expression in Soybean
- Root Biology Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Received date: 2021-02-13
Accepted date: 2021-05-07
Online published: 2021-05-07
摘要
共生根瘤的固氮效率受外界氮素的严格调控。除固氮酶活性外, 豆血红蛋白(Lb)浓度亦是反应固氮能力的重要指标。为明确氮水平对生物固氮作用的影响, 以大豆(Glycine max)为材料, 在低氮(0.53 mmol·L-1)条件下接种根瘤菌, 30天后再进行高氮(5.3、10、20、30和40 mmol·L-1)处理7天, 分析Lb浓度、固氮酶活性及类菌体发育状态。结果表明, 随着外界氮浓度的增加, 根瘤由红变绿, 且红色Lb明显减少而绿色Lb急剧增加; 固氮酶活性显著被抑制, 类菌体中侵染细胞数目和面积显著下降, 表明高氮引起Lb形态的改变与固氮能力关系密切。利用生物信息学及公开表达谱等数据进行分析, 发现大豆根瘤中主要含有4个共生Lb基因, 即GmLb1、GmLb2、GmLb3和GmLb4。4个GmLbs亲缘关系很近且位于进化树的同一分支。进一步分析GmLb1-4转录水平对氮的响应, 结果表明, GmLb1-4的表达显著受高氮抑制。研究结果可为揭示氮介导根瘤衰老机制及生物固氮的应用提供依据。
本文引用格式
杜梦柯, 连文婷, 张晓, 李欣欣 . 氮处理对大豆根瘤固氮能力及GmLbs基因表达的影响[J]. 植物学报, 2021 , 56(4) : 391 -403 . DOI: 10.11983/CBB21037
Abstract
The nitrogen fixation efficiency of symbiotic nodules is tightly regulated by external nitrogen (N). In addition to nitrogenase activity, the leghemoglobin (Lb) amount is vital index contributing to N2 fixation. To determine the effect of environmental N level on biological nitrogen fixation, soybean plants were inoculated with rhizobia for 30 d under low N condition (0.53 mmol·L-1), then transplanted to hydroponic culture solution with relatively higher N concentrations, including 5.3, 10, 20, 30, and 40 mmol·L-1 for 7 d, respectively. Lb concentration, nitrogenase activity and bacteroid development status were measured. Nodule displayed changed color from red to green with increasing N concentrations. Consistently, the red Lb concentration gradually declined with increased green Lb concentration. Moreover, the nitrogenase activity, infected cell number and area in nodules were all significantly decreased, suggesting that changes in Lb forms caused by excess N are closely associated with nitrogen fixation capacity. Bioinformatics and public expression profile data displayed that four symbiosis-associated Lbs, including GmLb1, GmLb2, GmLb3, and GmLb4 are the major Lb genes in soybean nodulation. These four GmLbs belong to the same clade of phylogenetic tree. Further analysis on the transcripts of GmLb1-4 in response to N showed that the expression levels of GmLb1-4 were all significantly inhibited by high N. This study services as a reference for future studies in understanding the underlying mechanisms of N-triggered nodule senescence as well as BNF application in agriculture.
Key words： biological nitrogen fixation; GmLbs; leghemoglobin; nitrogen concentration; soybean
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