60Co-γ射线辐照种子对牛至形态与挥发性成分的影响
收稿日期: 2021-01-18
录用日期: 2021-04-19
网络出版日期: 2021-04-21
基金资助
中国科学院A类战略性先导科技专项(XDA26040306);国家科技资源共享服务平台“国家重要野生植物种质资源库”(ZWGX2004)
Effects of 60Co-γ Ray Radiation on Morphology and Volatile Components of Origanum vulgare
Received date: 2021-01-18
Accepted date: 2021-04-19
Online published: 2021-04-21
牛至精油中的主要成分香芹酚和百里香酚具有较强的抑菌活性和替代抗生素的良好潜力。为了筛选有价值的遗传变异, 构建了牛至(Origanum vulgare)的60Co-γ射线育种体系, 利用固相微萃取联合气相色谱-质谱方法进行挥发性成分测定, 并通过主成分分析和层次聚类等多元分析法, 深入探讨60Co-γ射线对牛至M1代形态特征、腺毛密度和腺毛大小以及挥发性成分的影响。结果表明, 牛至种子的半致死剂量为16.39 Gy; 辐照M1代植株的株高、茎粗、分枝数、叶长和叶宽均发生变化, 筛选到多株形态突变体; 同时, 腺毛密度和腺毛大小发生多种变化, 从163株存活株中筛选出25株腺毛密度与大小均显著增大的单株;60Co-γ射线处理对牛至挥发性成分的种类影响相对较小, 主要影响挥发性成分的含量, 并可诱导5种化学型, 即香芹酚型、百里香酚型、γ-萜品烯型、β-石竹烯型和大根香叶烯型; 最终筛选获得6株香芹酚和百里香酚含量升高的突变单株。研究证实了60Co-γ射线辐照可作为牛至育种的一种有效诱变手段, 适宜辐照剂量为20 Gy。研究为牛至优良种质资源的选育提供了基础数据和新途径。
闫一皓, 王頔, 李静一, 张文颖, 郝渊鹏, 夏菲, 李慧, 白红彤, 石雷 . 60Co-γ射线辐照种子对牛至形态与挥发性成分的影响[J]. 植物学报, 2021 , 56(5) : 533 -543 . DOI: 10.11983/CBB21012
Carvacrol and thymol, the main components of essential oil in oregano (Origanum vulgare), have strong antibacterial activity, and have big potential to replace antibiotics. In order to acquire valuable genetic variation in oregano,60Co-γ ray radiation was utilized in breeding of oregano. The volatile components were analyzed by SPME-GC-MS, also principal component analysis and hierarchical clustering composition were performed. The effects of60Co-γ ray radiation on morphological characteristics were studied, including glandular hair density, glandular hair size and volatile components of M1 generation of oregano. The results showed that the LD50 of oregano seeds was 16.39 Gy; The plant height, stem diameter, branching number, leaf length and width of irradiated M1 generation were all changed, and several morphological mutants were selected; At the same time, the density and size of glandular hair changed in a variety of ways, and 25 mutants with significantly increase in density and size of glandular hair were selected from 163 surviving plants;60Co-γ ray treatment had little effect on volatile components, but significant effect on the content of volatile components, and 5 chemotypes in oregano were induced, including carvacrol-type, thymol-type, γ-terpinene-type, β-caryophyllene- type, and germacrene-type. Six mutants with high carvacrol and thymol were obtained. These results confirmed that60Co-γ ray radiation could be useful as an effective mutagenic method for oregano breeding, and the suitable radiation dose was 20 Gy. This study provided basic data and new way for the breeding and selection of new germplasm resources in oregano.
Key words： oregano; radiation breeding; gamma ray; carvacrol; thymol; volatile components
| [1] | 董燕梅, 张文颖, 凌正一, 李靖锐, 白红彤, 李慧, 石雷 (2020). 转录因子调控植物萜类化合物生物合成研究进展. 植物学报 55, 340-350. |
| [2] | 郝渊鹏, 李静一, 杨瑞, 李慧, 白红彤, 石雷 (2020). 芳香植物精油的抗菌性及在动物生产中的应用. 植物学报 55, 644-657. |
| [3] | 黄桂丹 (2016). 60Co-γ射线辐射育种研究进展. 林业与环境科学 32(2), 107-111. |
| [4] | 李好勋, 王国栋 (2015). 植物腺毛次生代谢产物生物合成的研究进展. 中国科学: 生命科学 45, 557-568. |
| [5] | 刘录祥, 郭会君, 赵林姝, 李军辉, 古佳玉, 赵世荣, 王晶 (2009). 植物诱发突变技术育种研究现状与发展前景. 核农学报 23, 1001-1007. |
| [6] | 王晶, 刘录祥, 赵世荣, 杨俊诚, 郭会君, 赵林姝, 陈文华 (2003). 7Li离子束诱变紫松果菊的生物效应研究初报. 核农学报 17, 405-408. |
| [7] | 余蓉培, 李杨, 李东, 詹选怀, 石雷 (2015). 荚果蕨绿色球状体对60Coγ射线的辐射敏感性. 植物学报 50, 565-572. |
| [8] | 昝俊峰, 陈艳霞, 陈平, 刘焱文, 刘军锋 (2013). 不同产地牛至挥发性成分的SPME/GC/MS法分析. 时珍国医国药 24, 37-39. |
| [9] | 张婷婷, 马嘉伟, 王路尧, 唐克轩, 李杉, 赵静雅 (2018). 过量表达黄烷酮3-羟化酶基因(AaF3H)提高青蒿中青蒿素的含量. 生物技术进展 8, 55-62. |
| [10] | Arabaci T, Çelenk S, Özcan T, Martin E, Yazici T, Açar M, Üzel D, Dirmenci T (2021). Homoploid hybrids of Origanum (Lamiaceae) in Turkey: morphological and molecular evidence for a new hybrid. Plant Biosyst Int J Deal Asp Plant Biol 155, 470-482. |
| [11] | Bouyahya A, Zengin G, Belmehdi O, Bourais I, Chamkhi I, Taha D, Benali T, Dakka N, Bakri Y (2020). Origanum compactum Benth., from traditional use to biotechnological applications. J Food Biochem 44, e13251. |
| [12] | Eilert U (1987). Elicitation: methodology and aspects of application. In: Constabel F, Vasil IK, eds. Cell Culture in Phytochemistry. Amsterdam: Elsevier Science. pp. 153-196. |
| [13] | Elshafie HS, Armentano MF, Carmosino M, Bufo SA, De Feo V, Camele I (2017). Cytotoxic activity of Origanum vulgare L. on hepatocellular carcinoma cell line HepG2 and evaluation of its biological activity. Molecules 22, 1435. |
| [14] | Esnault MA, Legue F, Chenal C (2010). Ionizing radiation: advances in plant response. Environ Exp Bot 68, 231-237. |
| [15] | Fan XT, Thayer DW, Sokora KJB (2004). Changes in growth and antioxidant status of alfalfa sprouts during sprouting as affected by gamma irradiation of seeds. J Food Prot 67, 561-566. |
| [16] | Fikry S, Khalil N, Salama O (2019). Chemical profiling, biostatic and biocidal dynamics of Origanum vulgare L. essential oil. AMB Express 9, 41. |
| [17] | Gavalas NP, Kalburtji KL, Kokkini S, Mamolos AP, Veresoglou DS (2011). Ecotypic variation in plant characteristics for Origanum vulgare subsp. hirtum populations. Biochem Syst Ecol 39, 562-569. |
| [18] | Jan S, Parween T, Siddiqi TO, Mahmooduzzafar (2012). Effect of gamma radiation on morphological, biochemical, and physiological aspects of plants and plant products. Environ Rev 20, 17-39. |
| [19] | Kazama Y, Ishii K, Hirano T, Wakana T, Yamada M, Ohbu S, Abe T (2017). Different mutational function of low- and high-linear energy transfer heavy-ion irradiation demonstrated by whole-genome resequencing of Arabidopsis mutants. Plant J 92, 1020-1030. |
| [20] | Koseki PM, Villavicencio ALCH, Brito MS, Nahme LC, Sebastião KI, Rela PR, Almeida-Muradian LB, Mancini- Filho J, Freitas PCD (2002). Effects of irradiation in medicinal and eatable herbs. Radiat Phys Chem 63, 681-684. |
| [21] | Kowalczyk A, Przychodna M, Sopata S, Bodalska A, Fecka I (2020). Thymol and thyme essential oil-new insights into selected therapeutic applications. Molecules 25, 4125. |
| [22] | Leyva-López N, Gutiérrez-Grijalva EP, Vazquez-Olivo G, Heredia JB (2017). Essential oils of oregano: biological activity beyond their antimicrobial properties. Molecules 22, 989. |
| [23] | Lukas B, Schmiderer C, Novak J (2015). Essential oil diversity of European Origanum vulgare L. (Lamiaceae). Phytochemistry 119, 32-40. |
| [24] | Majdi M, Malekzadeh-Mashhady A, Maroufi A, Crocoll C (2017). Tissue-specific gene-expression patterns of genes associated with thymol/carvacrol biosynthesis in thyme ( Thymus vulgaris L.) and their differential changes upon treatment with abiotic elicitors. Plant Physiol Bioch 115, 152-162. |
| [25] | Morshedloo MR, Mumivand H, Craker LE, Maggi F (2018). Chemical composition and antioxidant activity of essential oils in Origanum vulgare subsp. gracile at different phenological stages and plant parts. J Food Process Preserv 42, e13516. |
| [26] | Oladosu Y, Rafii MY, Abdullah N, Hussin G, Ramli A, Rahim HA, Miah G, Usman M (2016). Principle and application of plant mutagenesis in crop improvement: a review. Biotechnol Biotechnol Equip 30, 1-16. |
| [27] | Oniga I, Puscas C, Silaghi-Dumitrescu R, Olah NK, Sevastre B, Marica R, Marcus I, Sevastre-Berghian AC, Benedec D, Pop CE, Hanganu D (2018). Origanum vulgare ssp. vulgare: chemical composition and biological studies. Molecules 23, 2077. |
| [28] | Pan QF, Mustafa NR, Tang KX, Choi YH, Verpoorte R (2016). Monoterpenoid indole alkaloids biosynthesis and its regulation in Catharanthus roseus: a literature review from genes to metabolites. Phytochem Rev 15, 221-250. |
| [29] | Rajput JD, Bagul SD, Pete UD, Zade CM, Padhye SB, Bendre RS (2018). Perspectives on medicinal properties of natural phenolic monoterpenoids and their hybrids. Mol Divers 22, 225-245. |
| [30] | Sarrou E, Tsivelika N, Chatzopoulou P, Tsakalidis G, Menexes G, Mavromatis A (2017). Conventional breeding of Greek oregano ( Origanum vulgare ssp. hirtum) and development of improved cultivars for yield potential and essential oil quality. Euphytica 213, 104. |
| [31] | Seo HY, Kim JH, Song HP, Kim DH, Byun MW, Kwon JH, Kim KS (2007). Effects of gamma irradiation on the yields of volatile extracts of Angelica gigas Nakai. Radiat Phys Chem 76, 1869-1874. |
| [32] | Sharifi-Rad M, Varoni EM, Iriti M, Martorell M, Setzer WN, del Mar Contreras M, Salehi B, Soltani-Nejad A, Rajabi S, Tajbakhsh M, Sharifi-Rad J (2018). Carvacrol and human health: a comprehensive review. Phytother Res 32, 1675-1687. |
| [33] | Tang KX, Shen Q, Yan TX, Fu XQ (2014). Transgenic approach to increase artemisinin content in Artemisia annua L. Plant Cell Rep 33, 605-615. |
| [34] | Topuz A, Ozdemir F (2003). Influences of γ-irradiation and storage on the carotenoids of sun-dried and dehydrated paprika. J Agric Food Chem 51, 4972-4977. |
| [35] | Vardhan PV, Shukla LI (2017). Gamma irradiation of medicinally important plants and the enhancement of secondary metabolite production. Int J Radiat Biol 93, 967-979. |
/
| 〈 |
|
〉 |
首页