黄瓜苋科凝集素基因的表达分析与逆境调控
收稿日期: 2020-11-01
录用日期: 2021-03-01
网络出版日期: 2021-03-01
基金资助
陕西省自然科学基础研究计划(2020JQ-866);陕西省教育厅专项科研计划(20JK0590);广东省自然科学基金(2017A030310332);深圳大学新教师科研启动项目(2019078)
Expression of Amaranthin-like Lectins Gene and Responses to Abiotic Stresses in Cucumber
Received date: 2020-11-01
Accepted date: 2021-03-01
Online published: 2021-03-01
凝集素是一类具有特异性糖结合活性的蛋白质, 通常具有1个或多个非催化的糖结合结构域。凝集素在植物对病原菌的防御反应中发挥重要作用。由于其抗细菌、真菌、病毒和昆虫等的活性, 凝集素在农业和生物医药领域都具有很大的应用潜力。作为最小的凝集素家族之一, 苋科凝集素的研究较少。该文通过对重要经济作物黄瓜(Cucumis sativus)的基因组进行分析, 对16种苋科凝集素基因在黄瓜基因组中的分布和位置进行研究, 并分析相关基因的外显子/内含子组成。进一步通过启动子分析, 阐明了苋科凝集素基因对非生物胁迫的响应情况。最后, 通过实时荧光定量PCR, 检测了黄瓜中4种苋科凝集素基因对低温、高盐、干旱和ABA处理的响应情况。研究结果可为揭示苋科凝集素的生理功能及其在植物胁迫响应中的作用提供参考。
赵菲, 党刘毅, 魏敏惠, 刘春莹, 冷伟, 尚琛晶 . 黄瓜苋科凝集素基因的表达分析与逆境调控[J]. 植物学报, 2021 , 56(2) : 183 -190 . DOI: 10.11983/CBB20177
Lectins are globular proteins with carbohydrate-binding sites which enable them to specifically recognize and bind single or more particular carbohydrate structures. Previous studies have shown that lectins play critical roles in plant defense against their herbivores and pathogens. Due to their toxicity towards organisms like bacteria, fungi, viruses and insects, lectins are believed to have great potential in both agricultural and medical applications. As one of the smallest lectin families, there has been very limited research on amaranthin-like lectins. Here, we analyzed the distributions and intron/exon structures from 16 different amaranthin-like genes in the genome of cucumber, an important economic crop. To evaluate their responses against different stresses/plant hormones, promoter analysis was performed for all amaranthin-like genes. Finally, real-time quantitative PCR were performed on stress-treated plants to analyze the responses of amaranthin-like genes towards cold, salt, drought stresses and ABA treatment. This work provides valuable information for the study of physiological roles of amaranthin-like proteins and their involvement in plant defense.
Key words： abiotic stress; amaranthin-like lectins; promoter; protein domain
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